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Hepatocytes are essential for toxicology testing of pharmaceuticals in vitro. Hepatocyte strains such as HepG2, Huh7, THLE-2, PLC-PRF-5, and AML-12 are used. A group from the National Center for Child Health and Development has established a new immortalized hepatocytes  named HepaMN from from a liver associated with biliary atresia. Immortalization was performed by inoculation with CDK4, cyclin D1, and TERT.
https://www.nature.com/articles/s41598-020-73992-3

Characteristics of HepaMN strains:
Established from untransformed hepatocytes, and has normal liver function and diploid chromosomes,
Expression of albumin gene at the same level as HepaRG,
Showing the cell morphology of hepatocytes,
Induces stable cytochrome P450 3A4 (CYP3A4) and shows normal metabolic effects,
Efficiently proliferates and is stable even in very long passages,
As a result, succeeded in providing a new and useful hepatocyte strain for toxicology testing.

HepaMN can be effectively cultured by EMUKK-15

Existing Pfizer and Modelna vaccines for the new coronavirus (SARS-CoV-2) are mRNA type, but the following group is evaluating prototype vaccines using adeno-associated viral (AAV).
https://www.biorxiv.org/content/10.1101/2021.01.05.422952v3.full.pdf

Therapeutic gene transfer using AAV is recognized as a highly safe method, and Glybera, Luxturna, Zortensma etc. have already been approved by the FDA and EMA as gene therapeutics.
Vaccines using two types of AAV have been prototyped. AAVCOVID-19-1 (AC1) encodes a full-length S protein, which expresses an antigen S protein on the cell membrane of an infected cell. Another, AAVCOVID-19-3 (AC3), encodes part of the S protein, and the antigen takes the form of a secretory protein.

The evaluation was carried out with mice and nonhuman primates, and the immune response was retained for about 5 months with a single doze. There is no problem with storage at room temperature for one month, and vaccines are amenable to large scale production because the methodology has been established. As an effect, AC1 seems to be higher than AC3.

However, the blog Admin has some concerns that the antigen genes are incorporated into cells.

It has been pointed out that mutations in the new coronavirus (SARS-CoV-2) are behind infection explosions in the UK and South Africa. A number of mutations have occurred, but a mutation common to these has already been shown to be N501Y.
A group of The University of Texas Medical Branch, Galveston TX has reported on how the mutation affects the effects of Pfizer’s vaccine, Tozinameran (BNT162b2, a nucleoside modified RNA vaccine).
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7805448/

A comparative experiment using N501 and Y501, which is the genetic background of N501,  was done by measuring  50% plaque reduction neutralization titers for N501 and Y501 viruses using sera from 20 people collected 2 weeks and 4 weeks after administration of the vaccine. As a result, it was reported that almost the same neutralization titers were obtained.

NIST in the U.S. offers humanized IgG Type 1 mAb as a reference mAb. NS0 cells are used to manufacture this mAb. NS0 cell is a model cell line derived from non-secretory mouse myeloma, which is commercially used in biomedical research and the production of therapeutic proteins.
https://pubmed.ncbi.nlm.nih.gov/31591262/

The status of glycan addition is reported by comprehensively following the results of 103 evaluations conducted at 73 institutions around the world. It is likely to be useful as glycan modification information of reference mAb of IgG Type 1.

A group from The University of Colorado Boulder etc.  has simulated on the priority of vaccine administration for the new coronavirus (COVID-19).
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7743091/

Five age groups of vaccination were assumed, and simulated changing the combination of the following parameters: vaccine rollout speeds to the population (0.05% to 1%/day), infection rates (1.15, 1.5), vaccine efficacy (90%), and also the case that the effectiveness of the vaccine decreases with age (60 years old = 90% – > 80 years old = 50%).
The results vary depending on the conditions, and the best choice replaced between the case that prioritizes the over-60s and the one that prioritizes 20 ~ 59 year-olds. However, since the prerequisites are always changing in reality, overall, it would be a better choice to prioritize the over-60s .

On December 15, 2020, Blog Admin uploaded an article saying that the new coronavirus (SARS-CoV-2) is likely an artificial product, citing “The 2nd Yan Report”.

On January 15, 2021, the U.S. Department of State released a FACT Sheet titled Activity at the Wuhan Institute of Virology (WIV).
https://www.state.gov/fact-sheet-activity-at-the-wuhan-institute-of-virology/

In this article, the U.S. Department of State avoids saying that SARS-CoV-2 originated in WIV, but it does show three FACTs in WIV as follows.

1. In the fall of 2019, there were several researchers who showed similar pathology to COVID-19 were in WIV.
2. Since 2016, WIV has been studying bat coronaviruses including “RatG13” (96.2% similar to SARS-CoV-2).
3. Secret Chinese biological weapons researches were being conducted on WIV