Archive 22/8/14

A group from Division of Laboratory Diagnostics, Faculty of Pharmacy, Wroclaw Medical University, Poland, etc. has reported that O-Glycosylation changes in serum IgG3 could be a marker for inflammation development in advanced endometriosis.
https://www.mdpi.com/1422-0067/23/15/8087/htm

Structurally, human IgG N-linked glycans are typically biantennary complexes. The second N-glycosylation site is found in the VH and VL (heavy and light chain of variable regions, respectively) and has been observed in 15–25% of all serum IgG. The presence of glycans in the IgG Fab region may contribute to higher antibody stability and modulate antigen binding. For IgG3, apart from N-glycans present in the Fab and Fc regions, the presence of O-linked glycans in the hinge region is also observed.

In blood serum, about 10% of IgG3 polyclonal antibodies and about 13% of IgG3 monoclonal antibodies are considered to contain O-glycans. Each IgG3 molecule can contain up to three O-glycans linked to threonine residues in the triple repeat regions within the hinge region. Although the function of IgG O-glycosylation is still not fully understood, the structure of the hinge region is hypothesized to be able to protect the immunoglobulin from proteolytic cleavage, and may also help maintain the extended conformation and flexibility of IgG3.

In this study, it was examined whether O-glycans are expressed in serum IgG in advanced endometriosis, and also whether, additionally to the presence of biantennary N-glycans, there are also highly branched N-glycans in IgG, and if so, whether the degree of their expression is characteristic of advanced endometriosis.

For the analysis of serum IgG O-glycosylation and the expression of multi-antennary N-glycans, lectin-ELISA with lectins specific to O-glycans (MPL, VVL, and Jacalin) and highly branched N-glycans (PHA-L) was used. And also, isolated serum IgG, i-IgG, and native serum IgG, s-IgG, were examined as samples.

The results were striking. In the case of s-IgG, the clinical value was limited. In the case of i-IgG, however, a maximum high clinical value (AUC = 1) was obtained with all four lectins used, both when comparing women with advanced endometriosis to healthy women and healthy women to a non-endometriosis group.
While this research has shown that both the expression of O-glycans and highly branched N-glycans in IgG may have a potential application in the diagnostics of advanced endometriosis, at the present stage of research, these conclusions mainly concern IgG isolated from serum. This makes it difficult to apply this type of determination in routine diagnostics due to the laborious and time-consuming procedure of protein isolation and purification. Nevertheless, this direction of research seems to be promising, and the development of a simple and fast protein isolation procedure is required.

A group from Division of Medical Safety Science, National Institute of Health Sciences, Japan, etc. has reported that stratifin (SFN) and presepsin (P-SEP) could be used as prognostic biomarkers for severe COVID-19 progression.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188980/

COVID-19 is a respiratory disease with a wide range of manifestations, from asymptomatic to severe cases with ARDS.18 Although about 80% of patients with COVID-19 experience only mild or moderate symptoms and have a favorable prognosis, the remaining patients worsen to severe or critical stage, and their prognosis, largely driven by severe ARDS, is poor. Therefore, early detection of the severe COVID-19 cases is important.

In the present study, five biomarkers for lung injury, SP-D, KL-6, P-SEP, KAL and SFN were analyzed, all of which have been suggested to be related with ARDS or its typical histological pattern diffuse alveolar damage (DAD), in serum samples collected serially from patients with COVID-19. It was found for the first time that serum SFN was significantly elevated in patients with severe COVID-19 compared to patients with mild or moderate symptoms. SFN, as well as P-SEP which has been suggested as a biomarker for severe COVID-19.

Both serum SFN and P-SEP were obviously elevated at the pre-severe stage. The AUC values [95% CI] of these proteins in diagnosing the pre-severe stage were 0.83 [0.76–0.90] for SFN and 0.79 [0.69–0.89] for P-SEP. When the cutoff values of SFN and P-SEP for discriminating the pre-severe condition from the mild/moderate condition on COVID-19 patients were set at 0.81 ng/mL and 374 pg/mL, the diagnostic sensitivity and specificity were 81.5% and 70.1% for SFN, and 76.9% and 71.9% for P-SEP, respectively.

A group from School of Pharmaceutical Sciences, Peking University, Beijing, China, etc. has reported about mycorrhizal bacteria strains isolated from Polyporus umbellatus.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340266/

Fungal pathogens cause a range of serious plant diseases, such as Fusarium wilt, and are responsible for most of the diseases in agricultural ecosystems.
There are two main ways to deal with the effects of these diseases:
One is to develop disease-resistant plants, and
the other is to use chemical fungicide to control the spread of pathogens.
However, these two methods have the disadvantages of long cycle and drug resistance of pathogens. A practical and sustainable strategy for dealing with phytopathogenic fungal diseases is therefore the application of biological control agents (BCAs).

Several bacteria have been isolated from plants and fungi, most of which belong to the genera Bacillus and Pseudomonas, and it has been demonstrated that those genera exhibit satisfactory biological activity and marked biocontrol potential.

In this paper, 21 rhizobacteria strains were isolated from mycorrhizal samples of Polyporus umbellatus, and evaluated from viewpoints of agricultural sustainability without requiring the overuse of hazardous fungicide and plant growth promoting ability such as producing IAA, siderophore, etc. and dissolving phosphate.

Among those isolated strains, it was found that the Pseudomonas strain ZL8 shows the highest performance as plant growth-promoting bacteria (PGPB). Nineteen compounds were identified from the fermentation broth of the strain ZL8, of which 2,4-diacetylphloroglucinol (DAPG) showed a significant inhibitory effect on phytopathogenic fungi with a minimum inhibitory concentration of 3.12–25 μg/mL. The plant growth promoting ability was evaluated with using Salvia miltiorrhiza, and the effect was so significant as shown below.

where, Fo. means Fusarium oxysporum

A group from Department of Biomedical and Molecular Sciences, Queen’s University, Kingston, ON, Canada has reported that green tea catechin, epigallocatechin gallate (EGCG), could be a pan-coronavirus attachment inhibitor.
https://www.nature.com/articles/s41598-022-17088-0

Here, it was evaluated and characterized the inhibitory activity of EGCG against entry of human seasonal and highly pathogenic coronavirus (CoVs). Then, it was demonstrated that EGCG inhibits entry of a broad range of CoVs into physiologically relevant human lung epithelial cells. And, furthermore, EGCG inhibited binding of multiple human CoVs to cell surfaces, suggesting that this natural product inhibits a highly conserved step in CoV attachment, such as primary attachment to cell-surface heparan sulfate.
Focusing on SARS-CoV-2, it was shown that EGCG competitively inhibits virion attachment to heparin, a structural analog of Heparan Sulfate. These findings further support understanding of the antiviral mechanisms of EGCG against CoVs, and identify a highly conserved antiviral target for the development of improved antiviral molecules to prevent infection with diverse CoVs, including potential future emerging CoVs.