HLA-I and HLA-II antigens derived from SARS-CoV-2 

HLA-I and HLA-II antigens derived from SARS-CoV-2 

A group from Weizmann Institute of Science, Rehovot, Israel, etc. has reported on HLA-I and HLA-II antigens derived from SARS-CoV-2.
https://pubmed.ncbi.nlm.nih.gov/34166618/

HEK293T cell line stably expressing angiotensin-converting enzyme 2 (ACE2) and IHW01070, Calu-3 cell lines were infected with SARS-CoV-2. The, HLA peptidomics to profile HLA-I- and HLA-II-bound antigens was performed. All HLA peptidomics analyses were performed in triplicate.

We identified 10 different HLA-I peptides derived from canonical ORFs of the virus.
Two peptides were derived from the spike protein, NEVAKNLNESL and TGSNVFQTR,
Two peptides were derived from nsp3, STTTNIVTR and YYTSNPTTF,
One peptide was derived from ORF3, FTIGTVTLK,
One peptide was derived from nsp1, HSSGVTREL,
Four peptides were derived from nucleocapsid, APRITFGGP, RITFGGPSD, NAPRITFGGP and ITFGGPSDSTGSNQNGER.

A nested set of 25 peptides as HLA-II peptides was derived from membrane as shown below.

These information will help to develop vaccines.

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