A group from Department of Rheumatology, Shanghai Institute of Rheumatology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University School of Medicine, China, etc. has reported that urine Galectin-3 binding protein (uG3BP) is a potential biomarker of Lupus nephritis (LN).
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962213/
The levels of uG3BP were significantly increased in active LN patients compared to those in inactive LN (p<0.001), CKD patients (p=0.01), and healthy controls (p<0.001). ROC analysis indicated a good discrimination ability of uG3BP to differentiate active LN from chronic kidney diseases (CKD) patients (AUC=0.7), inactive LN (AUC=0.76), or healthy controls (AUC=0.87).
The uG3BP levels were measured in urine samples by ELISA assay using human Galectin-3BP/MAC-2BP ELISA kit (DY2226) from R&D Systems (Minneapolis, MN, USA) according to the manufacturer’s instructions. All urine samples were diluted 1:30. uG3BP levels were normalized by urine creatinine levels. Urine creatinine levels were measured by Creatinine Parameter Assay Kit (KGE005, R&D Systems, Minneapolis, MN) using the same samples.
