Agalacto type complex N-glycans without terminal Gal are often seen in autoimmune disease. Well known Lectins with glycan binding specificity to agalacto are BLL, PVL, GSL-II as shown below. BLL and PVL are mushroom derived, and GSL-II is bean derived.
Boletopsis leucomelaena: BLL lectin
Psathyrella velutina: PVL lectin
Griffonia simplicifolia: GSL-II lectin
A group from New England Biolabs, Inc. has identified gene sequence of Boletopsis grisea, a close North American relative of Boletopsis leucomelaena, and investigated glycan binding specificities of recombinant lectin (named rBGL) with using glycan arrays. Curiously, rBGL has bi-specific glycan binding characteristics, not only to agalacto type N-glycans but also to O-glycans with Thomsen–Friedenreich antigen (TF-antigen; Galβ1,3GalNAc-α-). Depending on applications, the binding specificity to O-glycan would be interesting.
https://www.nature.com/articles/s41598-020-80488-7