A group from Georgia State University, etc. has reported detailed binding characteristics of STL, PNA, and Jacalin to O-GalNAc with using chemoenzymatic modular assembly of O-GalNAc glycans.
https://www.nature.com/articles/s41467-021-23428-x
STL showed moderate binding to core 2 and 6 glycans presenting a terminal LacNAc motif (with/without further modifications) but not to core 1 and 3 structures.
PNA selectively bound to T antigen and core 2 glycans with an unmodified β1-3Gal branch.
Jacalin bound strongly to all core 3 and nearly all core 1 glycans (devoid of α2-6 sialylation) at the same level of the Tn antigen, but did not bind to any core 2, 4, or 6 structures.
