A group from University of Copenhagen, etc. has reported on site-specific O-glycan analysis of the new coronavirus (SARS-CoV-2).
https://www.biorxiv.org/content/10.1101/2021.02.03.429627v2
Authors used SARS-CoV-2 Spike proteins expressed in Drosophia and HEK 293 F cells. N-glycans were removed by PNGase F, and then Sialic acids were also removed by neuraminidase. Glycan analysis was done by using ETD/HCD LC-MS/MS. O-glycan modification in HEK 293 F is shown blow. Tn-antigen seems to be more expressed than Tn-antigen. There is no information about sialylation of T- and Tn-antigens.
