A group from University of Wisconsin-Madison, etc. has reported on the analysis of O-glycans expressed on the new coronavirus (SARS-CoV-2).
https://www.biorxiv.org/content/10.1101/2021.02.28.433291v1
In order to analyze O-glycans, N-glycans on SARS-CoV-2 spike protein expressed with HEK293 were removed by PNGase F treatment. Authors combined ion mobility spectrometry (TIMS) and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis.
As a result, it was found that the majority of the O-glycan modification was Sialyl-T-antigen.
