Lectin microarrays are extremely powerful in comparative glycan profiling analysis. Furthermore, by using the evanescent-field fluorescence excitation method for lectin microarray analysis, it is possible to monitor weak interactions between lectins and glycans non-destructively, making it possible to perform comparative glycan profiling at the world’s highest quality level.
For details of this technology, please refer to the following.
As of today, there are some lectin microarrays in the world whose manufacturers are unknown, but there are also several products that are manufactured and sold on a commercial basis (PSS, RayBiotech, etc.).
Therefore, I would like to compare their quality from published images refering to papers that have used lectin microarrays in the past few years.
In conclusion, it can be said that there is no lectin microarray with quality superior to LecChip.
LecChip made by GlycoTechnica（Currently LecChips are manufactured by PSS）
Lectin microarray made by RayBiotech (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10348014/)
Spot sizes are irregular,
Some spots are very small,
There are also irregular spots that are hollow and have ring shapes
Lectin microarray, unknown manufacturer (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6636412/)
Probably this was manufactured with a pin-type spotter,
Spot inside is heavily ununiform
Lectin microarray, unknown manufacturer (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8798758/)
Intense voids in the spots
Lectin microarray, unknown manufacturer (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9772556/)
Spot sizes are irregular,
Intense void in the spots,
Spot inside is strongly ununiform,
Some spots are probaly missing
Lectin microarray, unknown manufacturer (https://www.frontiersin.org/articles/10.3389/fchem.2021.637730/full)
Relatively acceptable except for voids in the spots,
Lectin microarray, unknown manufacturer (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840895/)
Spot sizes are irregular,
Spot inside is heavily ununiform
