A group from Laboratory of Functional Glycomics, College of Life Sciences, Northwest University, Xi’an, Shaanxi, China, etc. has reported about comparative glycan profiling between drug-sensitive Pseudomonas aeruginosa (DSPA) strains and carbapenem-resistant Pseudomonas aeruginosa (CRPA) strains.
https://pubmed.ncbi.nlm.nih.gov/37861315/
lectin microarrays were used to analyze the differences in glycan alterations between 53 drug-sensitive DSPA strains and 57 carbapenem-resistant CRPA strains obtained from clinical isolates, with the goal of identifying important glycopatterns associated with carbapenem resistance.
In this experiment, whole bacterial cell lysates, which were fluorescently labeled with Cy3, were applied onto Lectin microarrays to take glycan profiles of bacteria.
As a result, it was found that LCA could be an strong biomarker detecting differential expression of glycan structures on the bacterial surface between DSPA with CRPA.
