A group from University of Southampton, etc. has compared site-specific glycosylation of SARS-CoV-2 spike proteins (all recombinants) among five laboratories.
The cells used for Sproten expression were as follows.
HEK293： Amsterdam, Harvard,
HEK293F： Southampton/Texas,
HEK293T： Oxford,
CHO： Swiss,
It is celarly shown that site-specific glycosylation changes considerablly with reflecting differences in cells and culture conditions.
Fundamentally speaking, it is a mixture of oligo mannose and complex type N-glycans.
Blog admin is interested in how much these differences cause difference in the infectivity, how glycosylation changes with SARS-CoV-2 mutations, and how much the glycosylation changes due to mutations affect the infectivity.
https://www.biorxiv.org/content/10.1101/2021.03.08.433764v1.full
