{"id":9711,"date":"2022-08-21T07:59:21","date_gmt":"2022-08-20T22:59:21","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=9711"},"modified":"2022-08-21T08:02:45","modified_gmt":"2022-08-20T23:02:45","slug":"human-surfactant-protein-d-facilitates-sars-cov-2-binding-and-entry-in-dc-sign-expressing-cells","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/human-surfactant-protein-d-facilitates-sars-cov-2-binding-and-entry-in-dc-sign-expressing-cells\/","title":{"rendered":"Human surfactant protein D facilitates SARS-CoV-2 binding and entry in DC-SIGN expressing cells"},"content":{"rendered":"

A group from College of Health, Medicine and Life Sciences, Brunel University London, Uxbridge, UK, etc. has reported that human surfactant protein D (human SP-D) facilitates SARS-CoV-2 binding and entry in DC-SIGN expressing cells
\nhttps:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2022.960733\/full<\/a><\/p>\n

The ability of a recombinant fragment of human SP-D (rfhSP-D) to mediate the binding of SARS-CoV-2 to DC-SIGN expressing cells was evaluated. HEK 293T cells were transfected with a construct containing a DNA sequence of full-length human DC-SIGN to induce DC-SIGN cell surface expression (DC-HEK cells). To assess the effect of rfhSP-D on pseudotypes binding to DC HEK cells, the DC-HEK cells were challenged with rfhSP-D (20\u00b5g\/ml) treated SARS-CoV-2 Spike protein-expressing pseudotype. Increased binding (~50%) in the treated samples (DC-HEK + SARS-CoV-2 spike Pseudotypes + rfhSP-D) compared to their untreated counterparts (DC-HEK + SARS-CoV-2 spike Pseudotypes) was observed. Similar experiments were done using THP-1 cells treated with PMA and IL-4 to induce the expression of native DC-SIGN. rfhSP-D treatment was found to increase the binding efficiency of the pseudotypes to the THP-1 cells expressing DC-SIGN by ~25%, compared to the untreated controls.<\/p>\n

A blind docking approach was attempted to generate SARS-CoV-2 and DC-SIGN complexes. Analysis of the top ranked docked poses revealed that NTD (N-terminal domain) of spike protein interacted with the CRD domain of DC-SIGN. Tripartite complexes were generated by docking C-SIGN and SP-D with Spike protein. The top two docked poses (C1 and C2) were analysed for intermolecular interactions. In both C1 and C2 complexes, DC-SIGN (CRD) interacted with NTD domain of Spike protein. In C1, there were no molecular interactions between Spike protein and rfhSP-D. In C2, Spike protein interacted with rfhSP-D through RBD. <\/p>\n

As a conclusion, it was shown that SP-D interacts with RBD and DC-SIGN interacts with NTD of SARS-CoV-2 spike protein, and also SP-D stabilises DC-SIGN and SARS-CoV-2 spike protein interaction.<\/p>\n

\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

A group from College of Health, Medicine and Life Sciences, Brunel University London, Uxbridge, UK, etc. has r<\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70],"tags":[],"class_list":["post-9711","post","type-post","status-publish","format-standard","hentry","category-nature-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/9711","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=9711"}],"version-history":[{"count":1,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/9711\/revisions"}],"predecessor-version":[{"id":9712,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/9711\/revisions\/9712"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=9711"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=9711"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=9711"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}