{"id":9630,"date":"2022-08-14T08:00:14","date_gmt":"2022-08-13T23:00:14","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=9630"},"modified":"2022-08-14T08:23:15","modified_gmt":"2022-08-13T23:23:15","slug":"streptococcal-pharyngitis-detection-using-h-1-antigen-glycan-probe-conjugated-gold-nanoparticles","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/streptococcal-pharyngitis-detection-using-h-1-antigen-glycan-probe-conjugated-gold-nanoparticles\/","title":{"rendered":"Streptococcal Pharyngitis detection using H-1 antigen (glycan probe) conjugated Gold Nanoparticles"},"content":{"rendered":"<p>A group from Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran, etc. has reported about a Nanobiosensor based on H-1 antigen-Gold NanoParticles (AuNPs) aggregation detecting M1 Streptococcal Pharyngitis.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9354983\/\">https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9354983\/<\/a><\/p>\n<p>Streptococcus pyogenes (GAS) is a Gram positive bacterium that causes a wide variety of clinical conditions, ranging from acute pharyngitis to severe invasive diseases. A common method for the diagnosis of streptococcal pharyngitis is a physical examination by a physician and then a bacterial culture of throat swab. Until now, various types of biosensors were developed to diagnose GAS for clinical use. Those are based on antibody-antigen interactions, or on an isothermal nucleic acid amplification technology. Nevertheless, they are not widely used by clinicians worldwide, which may be related to the high cost.<\/p>\n<p>The nanobiosensors based on the aggregation of AuNPs are well-known, and have been studied as an easy-to-use diagnosis of pathogens, where the presence of a specific antigen in a colloidal medium containing bioreceptor\u2013AuNPs conjugates results in the aggregation of nanoparticles.\u00a0Since it is known that M1 GAS binds to H-1 antigen, in this study, a sugar code present on oral epithelial cells, Lacto-N-fucopentaose I-biotin (H-1-biotin) was conjugated on avidin-AuNPs as an antigen to detect M1 GAS.<\/p>\n<p><a href=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/08\/AuNP_red-shift.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-9626\" src=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/08\/AuNP_red-shift-300x249.png\" alt=\"\" width=\"300\" height=\"249\" srcset=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/08\/AuNP_red-shift-300x249.png 300w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/08\/AuNP_red-shift-600x497.png 600w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/08\/AuNP_red-shift-230x191.png 230w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/08\/AuNP_red-shift-350x290.png 350w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/08\/AuNP_red-shift-480x398.png 480w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/08\/AuNP_red-shift.png 694w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p>Based on the value of red-shift in SPR position, a calibration curve for detection of M1 GAS was plotted at a concentration range from 1 \u00d7 10<sup>2<\/sup> up to 1 \u00d7 10<sup>7<\/sup> CFU\/ml. It was found that M1 GAS was detected in a wide concentration range (1 \u00d7 10<sup>3<\/sup> \u2013 1\u00d710<sup>6<\/sup> CFU\/ml) with a linear response and a short detection time of 20 min.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A group from Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran, etc. has reported about<\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/streptococcal-pharyngitis-detection-using-h-1-antigen-glycan-probe-conjugated-gold-nanoparticles\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-9630","post","type-post","status-publish","format-standard","hentry","category-technology-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/9630","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=9630"}],"version-history":[{"count":5,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/9630\/revisions"}],"predecessor-version":[{"id":9636,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/9630\/revisions\/9636"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=9630"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=9630"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=9630"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}