{"id":8694,"date":"2022-05-02T06:00:22","date_gmt":"2022-05-01T21:00:22","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=8694"},"modified":"2022-04-30T07:30:52","modified_gmt":"2022-04-29T22:30:52","slug":"%e6%b0%b4%e4%bb%99%e3%82%bf%e3%82%bc%e3%83%83%e3%82%bf%e3%81%ae%e7%90%83%e6%a0%b9%e3%81%8b%e3%82%89%e6%8a%bd%e5%87%ba%e3%81%95%e3%82%8c%e3%81%9f%e6%96%b0%e3%81%97%e3%81%84%e3%83%ac%e3%82%af%e3%83%81","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/%e6%b0%b4%e4%bb%99%e3%82%bf%e3%82%bc%e3%83%83%e3%82%bf%e3%81%ae%e7%90%83%e6%a0%b9%e3%81%8b%e3%82%89%e6%8a%bd%e5%87%ba%e3%81%95%e3%82%8c%e3%81%9f%e6%96%b0%e3%81%97%e3%81%84%e3%83%ac%e3%82%af%e3%83%81\/","title":{"rendered":"\u6c34\u4ed9\u30bf\u30bc\u30c3\u30bf\u306e\u7403\u6839\u304b\u3089\u62bd\u51fa\u3055\u308c\u305f\u65b0\u3057\u3044\u30ec\u30af\u30c1\u30f3\uff08NTL-125\uff1a\u30de\u30f3\u30ce\u30fc\u30b9\u7d50\u5408\u578b\uff09\u304cSARS-CoV-2\u306e\u512a\u308c\u305f\u963b\u5bb3\u5264\u306b\u306a\u308a\u5f97\u308b"},"content":{"rendered":"<p>Division of Plant Biology, Bose Institute, P\/12 C.I.T. Scheme VII(M), Kolkata, 700054, India\u3089\u306e\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u6c34\u4ed9\u30bf\u30bc\u30c3\u30bf\u306e\u7403\u6839\u304b\u3089\u62bd\u51fa\u3055\u308c\u305f\u65b0\u3057\u3044\u30ec\u30af\u30c1\u30f3\uff08NTL-125\uff1a\u30de\u30f3\u30ce\u30fc\u30b9\u7d50\u5408\u578b\uff09\u304cSARS-CoV-2\u306e\u512a\u308c\u305f\u963b\u5bb3\u5264\u306b\u306a\u308a\u5f97\u308b\u3068\u3044\u3046\u3053\u3068\u3092Vero-E6\u7d30\u80de\u3092\u7528\u3044\u305fin vitro\u306e\u30a2\u30c3\u30bb\u30a4\u3067\u793a\u3057\u3066\u3044\u307e\u3059\u3002<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8988448\/\">https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8988448\/<\/a><\/p>\n<p>NTL-125\u3068\u540d\u4ed8\u3051\u3089\u308c\u305f\u6c34\u4ed9\u30bf\u30bc\u30c3\u30bf\u306e\u7403\u6839\u304b\u3089\u62bd\u51fa\u3055\u308c\u305f\u30e6\u30cb\u30fc\u30af\u306a\u30de\u30f3\u30ce\u30fc\u30b9\u7d50\u5408\u690d\u7269\u30ec\u30af\u30c1\u30f3\u306f\u3001Vero-E6\u7d30\u80de\u3092\u7528\u3044\u305fin vitro\u30a2\u30c3\u30bb\u30a4\u306b\u304a\u3044\u3066\u3001SARS-CoV-2\u306e\u611f\u67d3\u3092\u6709\u52b9\u306b\u963b\u5bb3\u3067\u304d\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002 Vero-E6\u7d30\u80de\u3092\u4f7f\u7528\u3057\u305fSARS-CoV-2\u30a6\u30a4\u30eb\u30b9\u4fb5\u5165\u309250\uff05\u6e1b\u5c11\u3055\u305b\u308b\u963b\u5bb3\u6fc3\u5ea6\uff08IC<sub>50<\/sub>\uff09\u306f\u3001\u7d040.8 \u00b5g\/mL\uff0850 nM\uff09\u3067\u3042\u308a\u3001Vero-E6\u7d30\u80de\u306b\u5bfe\u3059\u308bNLE-125\u306e\u7d30\u80de\u6bd2\u6027\u306e\u30a2\u30c3\u30bb\u30a4\u306f\u30015 \u00b5g\/mL\u306795\uff05\u4ee5\u4e0a\u300110 \u00b5g\/mL\u306e\u6fc3\u5ea6\u306785\uff05\u4ee5\u4e0a\u306e\u751f\u5b58\u7387\u3092\u793a\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>\u5206\u5b50\u30c9\u30c3\u30ad\u30f3\u30b0\u6cd5\u306b\u3088\u308a\u3001RBD\u306e36\u6b8b\u57fa\u3068NTL-125\u306e44\u6b8b\u57fa\u304c\u7d50\u5408\u72b6\u614b\u3067\u4e92\u3044\u306b5\u00c5\u306e\u8ddd\u96e2\u5185\u306b\u3042\u308b\u306e\u306b\u5bfe\u3057\u3001ACE-2\u306e27\u6b8b\u57fa\u306fRBD\u306e32\u6b8b\u57fa\u306b\u8fd1\u63a5\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002ACE-2\u3068NTL-125\u306e\u4e21\u65b9\u306b\u76f8\u4e92\u4f5c\u7528\u3059\u308bRBD\u306e17\u306e\u6b8b\u57fa\u6570\u306f17\u500b\u3067\u3042\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u3059\u3079\u3066\u306e\u6b8b\u57fa\u306e\u4e2d\u3067\u3001RBD\u3068NTL-125\u8907\u5408\u4f53\u306e\u5834\u5408\u306b\u306f\u3001RBD\u306e10\u500b\u3068NTL-125\u306e11\u500b\u304c\u7d50\u5408\u72b6\u614b\u30673\u00c5\u306e\u8ddd\u96e2\u5185\u306b\u3042\u308a\u3001RBD\u3068ACE-2\u8907\u5408\u4f53\u306e\u5834\u5408\u306b\u306f\u3001RBD\u306e9\u6b8b\u57fa\u3068ACE-2\u306e9\u6b8b\u57fa\u304c3\u00c5\u306e\u8ddd\u96e2\u5185\u306b\u3042\u308a\u307e\u3057\u305f\u3002NTL-125\u306f\u3001ACE2\u304c\u901a\u5e38\u7d50\u5408\u3059\u308bSpike\u30bf\u30f3\u30d1\u30af\u8cea\u306eRBM\u3068\u307e\u3063\u305f\u304f\u540c\u3058\u9818\u57df\u3092\u5360\u3081\u3066\u3044\u307e\u3059\u3002 \u7d50\u679c\u3068\u3057\u3066\u3001NTL125-Spike\u30bf\u30f3\u30d1\u30af\u8cea\u76f8\u4e92\u4f5c\u7528\u306e\u7d50\u5408\u81ea\u7531\u30a8\u30cd\u30eb\u30ae\u30fc\u5909\u5316\u306f\u3001-13.3 kcal\/mol\u3001kd ~0.41nM\u3068\u306a\u308a\u3001ACE2-Spike\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7d50\u5408\u81ea\u7531\u30a8\u30cd\u30eb\u30ae\u30fc\u5909\u5316\uff08-11.2 kcal\/mol\u3001kd ~12 nM\uff09\u3088\u308a\u3082\u5927\u304d\u304f\u3001\u5f93\u3063\u3066\u524d\u8005\u304c\u5f8c\u8005\u3088\u308a\u3082\u5b89\u5b9a\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u660e\u78ba\u306b\u793a\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<p>\u66f4\u306b\u3001\u5206\u5b50\u30c9\u30c3\u30ad\u30f3\u30b0\u6cd5\u306b\u3088\u308a\u3001NTL-125-Spike\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u306e\u76f8\u4e92\u4f5c\u7528\u306f\u3001Spike\u30bf\u30f3\u30d1\u30af\u8cea\u306eAsn165\u306b\u7d50\u5408\u3057\u3066\u3044\u308b\u7cd6\u9396\u304cNTL-125\u306eIle137\u304a\u3088\u3073Thr138\u3068\u76f8\u4e92\u4f5c\u7528\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3082\u5a92\u4ecb\u3055\u308c\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u307e\u3057\u305f\u3002\u5373\u3061\u3001NTL-125-Spike\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u306e\u76f8\u4e92\u4f5c\u7528\u306f\u3001\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u3060\u3051\u3067\u306a\u304f\u3001\u7cd6\u9396\u90e8\u5206\u3082\u4ecb\u3057\u3066\u884c\u308f\u308c\u3066\u3044\u308b\u306e\u3067\u3059\u3002<\/p>\n<p><a href=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/05\/NTL-125_ACE2-glycan.png\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/05\/NTL-125_ACE2-glycan-300x243.png\" alt=\"\" width=\"300\" height=\"243\" class=\"alignnone size-medium wp-image-8699\" srcset=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/05\/NTL-125_ACE2-glycan-300x243.png 300w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/05\/NTL-125_ACE2-glycan-600x485.png 600w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/05\/NTL-125_ACE2-glycan.png 721w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Division of Plant Biology, Bose Institute, P\/12 C.I.T. Scheme VII(M), Kolkata, 700054, India\u3089\u306e\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u6c34\u4ed9\u30bf\u30bc\u30c3\u30bf\u306e\u7403\u6839\u304b<\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/%e6%b0%b4%e4%bb%99%e3%82%bf%e3%82%bc%e3%83%83%e3%82%bf%e3%81%ae%e7%90%83%e6%a0%b9%e3%81%8b%e3%82%89%e6%8a%bd%e5%87%ba%e3%81%95%e3%82%8c%e3%81%9f%e6%96%b0%e3%81%97%e3%81%84%e3%83%ac%e3%82%af%e3%83%81\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[642,42],"tags":[],"class_list":["post-8694","post","type-post","status-publish","format-standard","hentry","category-nature-ja","category-technology-ja"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/8694","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=8694"}],"version-history":[{"count":3,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/8694\/revisions"}],"predecessor-version":[{"id":8700,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/8694\/revisions\/8700"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=8694"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=8694"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=8694"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}