{"id":8415,"date":"2022-03-30T12:39:44","date_gmt":"2022-03-30T03:39:44","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=8415"},"modified":"2022-03-30T12:47:11","modified_gmt":"2022-03-30T03:47:11","slug":"monoclonal-antibody-reference-standard-with-extensive-glycan-characterization-nistmab","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/monoclonal-antibody-reference-standard-with-extensive-glycan-characterization-nistmab\/","title":{"rendered":"Monoclonal antibody reference standard with extensive glycan characterization: NISTmAb"},"content":{"rendered":"

Global Biologics, Science Division, United States Pharmacopeia, Rockville, MD 20852, USA has disclosed a monoclonal antibody reference standard, with extensive glycan characterization.
\nhttps:\/\/www.mdpi.com\/1424-8247\/15\/3\/315<\/a><\/p>\n

A monoclonal antibody reference standard, with extensive glycan characterization is available from the National Institute of Standards and Technology designated as NIST Reference Material 8671, NISTmAb.
\nThe United States Pharmacopeia (USP) developed three monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS<\/strong>) that may be used as control materials to demonstrate whether glycan characterization procedures provide an accurate result.
\nThe USP mAb reference standards are different proteins of the same IgG type 1 subclass<\/strong> yet provide sufficient variability to examine a broad spectrum of glycan structures. Glycan characterization is commonly performed by analysis of glycans released from the protein backbone.<\/p>\n

\"\"<\/a>
\nwhere,
\nF(6)A2<\/strong>: core fucosylated biantennary,
\nF(6)A2B<\/strong>: core fucosylated biantennary with bisecting GlcNAc,
\nM5<\/strong>: five mannose on core GlcNAc,
\nF(6)A1G(4)1<\/strong>: core fucosylated monoantennary with \u03b21-4 linked Galactose,
\nA2[6]G(4)1<\/strong>: biantennary with \u03b21-4 linked Galactose attached to \u03b11-6 linked core mannose,
\nF(6)A2[6]G(4)1<\/strong>: core fucosylated biantennary with \u03b21-4 linked Galactose attached to \u03b11-6 linked core mannose,
\nF(6)A2[3]G(4)1<\/strong>: core fucosylated biantennary with \u03b21-4 linked Galactose attached to \u03b11-3 linked core mannose,
\nF(6)A2[6]G(4)1Ga1<\/strong>: fucosylated bianntennary with a \u03b21,4-linked galactose directly attached to the \u03b11,6-linked core mannose, and an \u03b11,3-linked galactose attached to the \u03b21,4-linked galactose,
\nF(6)A2G(4)2<\/strong>: equal to G2F,<\/p>\n","protected":false},"excerpt":{"rendered":"

Global Biologics, Science Division, United States Pharmacopeia, Rockville, MD 20852, USA has disclosed a monoc<\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-8415","post","type-post","status-publish","format-standard","hentry","category-technology-en"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t