{"id":8146,"date":"2022-03-04T06:10:31","date_gmt":"2022-03-03T21:10:31","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=8146"},"modified":"2022-03-04T06:37:49","modified_gmt":"2022-03-03T21:37:49","slug":"glycan-binding-specificities-of-the-sars-cov-2-spike-protein","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/glycan-binding-specificities-of-the-sars-cov-2-spike-protein\/","title":{"rendered":"Glycan binding specificities of the SARS-CoV-2 spike protein"},"content":{"rendered":"

A group from Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan, etc. has reported about glycan binding specificities of the SARS-CoV-2 spike protein.
\nhttps:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8880561\/<\/a><\/p>\n

Assay:<\/strong>
\nDonor beads (500 ng\/well) and biotin-polyacrylamide (PAA)-sugars (20 ng\/well) mixed with SARS-CoV-2 spike protein S1 or S2 subunits (10\u201320 ng\/well) were incubated at room temperature for 1 h. A mixture of acceptor beads (500 ng\/well) and rabbit antisheep IgG Fc antibodies (10 ng\/well) was added to the reaction to reach a final volume of 25 \u03bcL. All reactions were performed in the dark. After incubation for 2 h, the binding signals were measured and analyzed using the AlphaScreenTM<\/strong><\/sup> detection program.<\/p>\n

Results:<\/strong>
\nIt was found that the SARS-CoV-2 spike protein S1 subunit binds specifically to blood group A antigen (strongly) and B antigen (weakly), and that the spike protein S2 subunit has a binding preference for Lewisa<\/sup> antigen. However, in the eyes of blog admin, it does not mean that SARS-CoV-2 Spike proteins have no affinity for glycan structures other than these ones, and the specificity is not so remarkable, especially in S2 subunit.<\/p>\n

\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

A group from Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng K<\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70],"tags":[],"class_list":["post-8146","post","type-post","status-publish","format-standard","hentry","category-nature-en"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t