{"id":7316,"date":"2022-01-04T10:56:21","date_gmt":"2022-01-04T01:56:21","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=7316"},"modified":"2022-01-04T10:58:42","modified_gmt":"2022-01-04T01:58:42","slug":"serum-glycobiomarkers-for-colorectal-carcinoma-using-reverse-phase-lectin-microarray-the-combination-of-phae-and-hl-sel-was-the-best","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/serum-glycobiomarkers-for-colorectal-carcinoma-using-reverse-phase-lectin-microarray-the-combination-of-phae-and-hl-sel-was-the-best\/","title":{"rendered":"Serum glycobiomarkers for colorectal carcinoma using reverse-phase Lectin Microarray: The combination of PHAE and HL sel was the best"},"content":{"rendered":"

A group from Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia, etc. has reported that the combination of lectins (PHAE + HL sel) can detect only glycosylation changes associated with colorectal cancer (CRC) and not with age.
\nhttps:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8695905\/<\/a><\/p>\n

Here, reverse-phase lectin microarrays were used for the identification of changes in the serum glycome as potential CRC biomarkers.<\/p>\n

What is the reverse-phase lectin microarrays:<\/strong>
\nSerum samples diluted 50\u00d7 were spotted in different wells in triplicates using a microarray spotter on epoxide-coated slides. Subsequently, after spotting and blocking the slides, 70 \u03bcl of biotinylated lectins (5 \u03bcg\/ml in PBS) was added and incubated at RT for 1 h. The slides were washed gently three times with PBS, and then 70 \u03bcl of streptavidin-fluorescent material conjugate (0.1 \u03bcg\/ml in PBS) was added for 15 min. After a washing step and additional wash with deionized water, fluorescence intensity was read at 635 nm using a microarray reader. <\/p>\n

Lectins used in this experiment:<\/strong>
\nAAL, RPL-Fuc1, PHAE, PHAL, ConA, DBA, WFL, WGA, RCAI, MAA, P sel, RPL-Sia2, SNAI, HPyL, HE sel, and HL sel<\/p>\n

The lectin combination PHAE (specific to N-ghlycans with outer Gal and bisecting GlcNAc) + HL sel (specific to 6-O-Su sLex<\/sup>) provided the highest discrimination accuracy based on the AUC value (0.989).<\/p>\n","protected":false},"excerpt":{"rendered":"

A group from Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia, etc. has reported that <\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-7316","post","type-post","status-publish","format-standard","hentry","category-technology-en"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t