{"id":6946,"date":"2021-11-26T10:11:09","date_gmt":"2021-11-26T01:11:09","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=6946"},"modified":"2021-11-26T11:36:11","modified_gmt":"2021-11-26T02:36:11","slug":"ace2-and-bsg-cd147-are-involved-in-sars-cov-2-infection-of-human-ips-cell-derived-podocytes","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/ace2-and-bsg-cd147-are-involved-in-sars-cov-2-infection-of-human-ips-cell-derived-podocytes\/","title":{"rendered":"ACE2 and BSG\/CD147 are involved in SARS-CoV-2 infection of human iPS cell-derived podocytes"},"content":{"rendered":"

A group from Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA, etc. has reported that ACE2 and BSG\/CD147 are involved in SARS-CoV-2 infection of human iPS cell-derived podocytes.
\nhttps:\/\/pubmed.ncbi.nlm.nih.gov\/34816259\/<\/a><\/p>\n

Human iPS cell-derived\u3000podocytes express many host factor genes (including ACE2, BSG\/CD147, TMPRSS2, CTSL, CD33, DC-SIGN\/CD209, SIGLEC9, SIGLEC10, ACTR3, CLEC10A) associated with SARS-CoV-2 binding and viral processing. It is known that proteases such as Transmembrane Serine Protease 2 (TMPRSS2) or cathepsin L (CTSL) promote fusion and internalization of the receptorviral spike complex. Human iPS cell-derived podocytes expressed lower levels of ACE2 and TMPRSS2 when compared to Calu-3, and both BSG\/CD147 and CTSL were expressed at high levels as shown below. <\/p>\n

\"\"<\/a><\/p>\n

Nevertheless, podocytes derived from human iPS cells were directly infected with SARS-CoV-2 at low Multiplicity of Infection (MOIs) of 0.01 to 1, and intriguingly, there was significantly more viral uptake in the podocytes than Calu3 (p-value < 0.0001) and Caco-2 cells (p-value < 0.0001). Pre-treatment of infected podocytes with both anti-ACE2 and anti-BSG\/CD147\nantibodies at a concentration as low as 0.1 \u00b5g\/ml significantly diminished viral uptake (p-value < 0.0001), suggesting that both ACE2 and BSG\/CD147 are involved in SARS-CoV2 internalization in human iPS cell-derived podocytes.\n<\/p>\n","protected":false},"excerpt":{"rendered":"

A group from Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, U<\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70],"tags":[],"class_list":["post-6946","post","type-post","status-publish","format-standard","hentry","category-nature-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/6946","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=6946"}],"version-history":[{"count":2,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/6946\/revisions"}],"predecessor-version":[{"id":6953,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/6946\/revisions\/6953"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=6946"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=6946"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=6946"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}