{"id":6823,"date":"2021-11-15T11:54:37","date_gmt":"2021-11-15T02:54:37","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=6823"},"modified":"2021-11-15T11:57:10","modified_gmt":"2021-11-15T02:57:10","slug":"changes-in-n-glycosylation-on-neuroblastoma-affect-on-cell-proliferation-and-invasion","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/changes-in-n-glycosylation-on-neuroblastoma-affect-on-cell-proliferation-and-invasion\/","title":{"rendered":"Changes in N-glycosylation on neuroblastoma affect on cell proliferation and invasion"},"content":{"rendered":"

A group from Department of Biochemistry and Molecular Biology, East Carolina University, Greenville, North Carolina, USA, etc. has reported that changes in N-glycosylation on neuroblastoma affect on cell proliferation and invasion.
\nhttps:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0259743<\/a><\/p>\n

Two types of neuroblastoma cells were used. One is an \u200bengineered N-glycosylation mutant cell line with Mgat1 knockdown, NB_1(-Mgat1), expressed solely oligomannose N-glycans, and the other was its parental cell line, NB_1 expressed significant levels of higher order N-glycans, complex and hybrid N-glycans as shown below (GNL and ConA have higher affinity to oligomannose type N-glycans, and E-PHA and L-PHA have higher affinity to complex type N-glycans).<\/p>\n

\"\"<\/a><\/p>\n

Cell growth was faster in NB_1 cells with complex type N-glycans than in NB_1(-Mgat1) cells without complex type N-glycans. Quantitatively speaking, there was about a 54% decrease in cell proliferation between NB_1(-Mgat1) cells and NB_1 cells.<\/p>\n

NB_1(-Mgat1) cells had more and longer protrusions than NB_1 cells, indicating that the glycosylation mutant cells solely expressing oligomannose type N-glycans were more invasive than those expressing complex types of N-glycans. To quantify cell invasiveness, the ratio of measured invasive area to the measured sphere area was measured. The cell invasiveness of the NB_1(-Mgat1) cell line was 2.3-fold greater than the NB_1 cell line.<\/p>\n","protected":false},"excerpt":{"rendered":"

A group from Department of Biochemistry and Molecular Biology, East Carolina University, Greenville, North Car<\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70],"tags":[],"class_list":["post-6823","post","type-post","status-publish","format-standard","hentry","category-nature-en"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t