{"id":6810,"date":"2021-11-14T08:31:31","date_gmt":"2021-11-13T23:31:31","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=6810"},"modified":"2021-11-14T08:52:48","modified_gmt":"2021-11-13T23:52:48","slug":"two-types-of-multivalent-lectins-produced-from-hemagglutinins-of-clostridium-botulinum-gg-binds-to-gal-galnac-and-rn-binds-to-neu5ac","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/two-types-of-multivalent-lectins-produced-from-hemagglutinins-of-clostridium-botulinum-gg-binds-to-gal-galnac-and-rn-binds-to-neu5ac\/","title":{"rendered":"Two types of multivalent lectins produced from hemagglutinins of clostridium botulinum: Gg binds to Gal\/GalNAc and Rn binds to Neu5Ac"},"content":{"rendered":"

A group from Tokyo University of Agriculture and Technology, Tokyo, 183-8509, Japan, etc. has reported two types of multivalent lectins produced from hemagglutinins of clostridium botulinum.
\nhttps:\/\/www.nature.com\/articles\/s41598-021-01501-1<\/a><\/p>\n

Clostridium botulinum is a gram-positive anaerobic bacterium that produces a neurotoxin. Botulinum bacteria are classified into serotypes A to G depending on the antigenicity of the toxin produced. The type C 16S progenitor toxin is composed of a neurotoxin, non-toxic non-hemagglutinin component and several hemagglutinin proteins (HA) designated as HA1, HA2, HA3a and HA3b. <\/p>\n

It has been know that type C HA1 has two binding sites: site I and site II. Site I binds to N-acetylneuraminic acid (Neu5Ac), N-acetylgalactosamine (GalNAc) and galactose (Gal) while site II binds to galactose only.<\/p>\n

Authors have developed two new multivalent lectins from the above mentioned hemagglutinins of clostridium botulinum designed to have different binding specificities were produced: namely Gg and Rn:
\nGg is an Alexa 488 labeled complex with HA1 WADF -HA2 WT-HA3 WT, which binds to Gal\/GalNAc,<\/p>\n

\"\"<\/a><\/p>\n

and, Rn is an Alexa 594 labeled complex with HA1 NQAA-HA2 WT-HA3 WT, which binds to Neu5Ac only.<\/p>\n

\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

A group from Tokyo University of Agriculture and Technology, Tokyo, 183-8509, Japan, etc. has reported two typ<\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-6810","post","type-post","status-publish","format-standard","hentry","category-technology-en"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t