{"id":6675,"date":"2021-10-27T11:43:46","date_gmt":"2021-10-27T02:43:46","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=6675"},"modified":"2021-10-27T11:46:04","modified_gmt":"2021-10-27T02:46:04","slug":"dual-inhibition-of-tmprss2-and-cathepsin-b-is-effective-in-preventing-sars-cov-2-infection-in-ace2-ips-cells","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/dual-inhibition-of-tmprss2-and-cathepsin-b-is-effective-in-preventing-sars-cov-2-infection-in-ace2-ips-cells\/","title":{"rendered":"Dual inhibition of TMPRSS2 and Cathepsin B is effective in preventing SARS-CoV-2 infection in ACE2-iPS cells"},"content":{"rendered":"<p>A group from Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan has reported that dual inhibition of TMPRSS2 and Cathepsin B is effective in preventing SARS-CoV-2 infection.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8527102\/pdf\/main.pdf\">https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8527102\/pdf\/main.pdf<\/a><\/p>\n<p>iPS cells that stably express ACE2 were used for this experiment, and TMPRSS2 and CTSB (Cathepsin B gene) were suppressed to about 1% or less, by using a CRISPER interference system. <\/p>\n<p>It was found that CTSB and TMPRSS2 are required for SARS-CoV-2 to infect ACE2-iPS cells. It is known that TMPRSS2 is present in the cell membrane and CTSB in endosomes, suggesting TMPRSS2 and CTSB play important roles in endocytosis independent and endocytosis-dependent infection, respectively.<\/p>\n<p>From this experiment, it was shown that Double-knockdown of TMPRSS2 and CTSB reduced the viral load to 0.036\u00b10.021%.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A group from Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan has reported <\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/dual-inhibition-of-tmprss2-and-cathepsin-b-is-effective-in-preventing-sars-cov-2-infection-in-ace2-ips-cells\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70,7],"tags":[],"class_list":["post-6675","post","type-post","status-publish","format-standard","hentry","category-nature-en","category-technology-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/6675","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=6675"}],"version-history":[{"count":1,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/6675\/revisions"}],"predecessor-version":[{"id":6676,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/6675\/revisions\/6676"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=6675"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=6675"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=6675"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}