{"id":6495,"date":"2021-10-15T10:28:38","date_gmt":"2021-10-15T01:28:38","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=6495"},"modified":"2021-10-15T10:31:35","modified_gmt":"2021-10-15T01:31:35","slug":"differences-in-sars-cov-2-spike-glycosylation-between-cho-and-hek-cells-oligomannose-increases-in-hek-cells","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/differences-in-sars-cov-2-spike-glycosylation-between-cho-and-hek-cells-oligomannose-increases-in-hek-cells\/","title":{"rendered":"Differences in SARS-CoV-2 Spike glycosylation between CHO and HEK cells: Oligomannose increases in HEK cells"},"content":{"rendered":"<p>A group from Center for Biologics Evaluation and Research, Food and Drug Administration, Baltimore, MD, USA, etc. has reported on differences in SARS-CoV-2 Spike glycosylation between CHO and HEK cells.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8497748\/pdf\/fchem-09-735558.pdf\">https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8497748\/pdf\/fchem-09-735558.pdf<\/a><\/p>\n<p>CHO-Spike exhibits more complex (mainly bi-antennary) and higher sialylation (\u03b12,3-linked), while HEK-Spike exhibits more high-mannose and a small amount of \u03b12,3-and \u03b12,6-linked sialic acids as follows.<\/p>\n<p><a href=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/10\/SARS-CoV-2-Spike_CHO-vs-HEK.png\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/10\/SARS-CoV-2-Spike_CHO-vs-HEK-300x170.png\" alt=\"\" width=\"300\" height=\"170\" class=\"alignnone size-medium wp-image-6489\" srcset=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/10\/SARS-CoV-2-Spike_CHO-vs-HEK-300x170.png 300w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/10\/SARS-CoV-2-Spike_CHO-vs-HEK-600x341.png 600w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/10\/SARS-CoV-2-Spike_CHO-vs-HEK-1024x581.png 1024w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/10\/SARS-CoV-2-Spike_CHO-vs-HEK-768x436.png 768w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/10\/SARS-CoV-2-Spike_CHO-vs-HEK.png 1143w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A group from Center for Biologics Evaluation and Research, Food and Drug Administration, Baltimore, MD, USA, e<\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/differences-in-sars-cov-2-spike-glycosylation-between-cho-and-hek-cells-oligomannose-increases-in-hek-cells\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70,7],"tags":[],"class_list":["post-6495","post","type-post","status-publish","format-standard","hentry","category-nature-en","category-technology-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/6495","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=6495"}],"version-history":[{"count":1,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/6495\/revisions"}],"predecessor-version":[{"id":6496,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/6495\/revisions\/6496"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=6495"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=6495"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=6495"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}