{"id":6325,"date":"2021-10-02T08:56:25","date_gmt":"2021-10-01T23:56:25","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=6325"},"modified":"2021-10-04T11:27:22","modified_gmt":"2021-10-04T02:27:22","slug":"a-highly-potent-and-broad-neutralization-antibody-against-sars-cov-2-was-screened","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/a-highly-potent-and-broad-neutralization-antibody-against-sars-cov-2-was-screened\/","title":{"rendered":"A highly potent and broad neutralization antibody against SARS-CoV-2 was screened"},"content":{"rendered":"

A group from Vanderbilt University Medical Center, Nashville, USA, etc. has reported that a highly potent and broad neutralization antibody against SARS-CoV-2 was screened using a LIBRA-seq technology.
\nhttps:\/\/pubmed.ncbi.nlm.nih.gov\/34592170\/<\/a><\/p>\n

To identify SARS-CoV-2 S-directed antibodies, authors utilized LIBRA-seq<\/strong>, a technology that enables high-throughput simultaneous determination of B cell receptor sequence and antigen reactivity at the single-cell level, expediting the process of lead candidate selection and characterization. Antigen-specific B cells were isolated from a donor with potently neutralizing antibodies in serum 3 months after infection confirmed by nasal swab RT-PCR testing for SARS-CoV-2. <\/p>\n

Of the 73 IgG+ B cells with high LIBRA-seq scores for SARS-CoV-2 Spike, nine lead candidates were selected for characterization as recombinant monoclonal antibodies.
\nFrom VSV assays, antibody named 54042-4 showed the best potency, at a IC50<\/sub> of 9 ng\/mL, and broad neutralization against existing SARS-CoV-2 variants.
\nIt was al so found that the 54042-4 heavy chain binds to RBD residues 443\u2013447 and the 54042-4 light chain contacts RBD residues 498\u2013500 from cryo-EM structure analysis.<\/p>\n

\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

A group from Vanderbilt University Medical Center, Nashville, USA, etc. has reported that a highly potent and <\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-6325","post","type-post","status-publish","format-standard","hentry","category-technology-en"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t