{"id":6325,"date":"2021-10-02T08:56:25","date_gmt":"2021-10-01T23:56:25","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=6325"},"modified":"2021-10-04T11:27:22","modified_gmt":"2021-10-04T02:27:22","slug":"a-highly-potent-and-broad-neutralization-antibody-against-sars-cov-2-was-screened","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/a-highly-potent-and-broad-neutralization-antibody-against-sars-cov-2-was-screened\/","title":{"rendered":"A highly potent and broad neutralization antibody against SARS-CoV-2 was screened"},"content":{"rendered":"<p>A group from Vanderbilt University Medical Center, Nashville, USA, etc. has reported that a highly potent and broad neutralization antibody against SARS-CoV-2 was screened using a LIBRA-seq technology.<br \/>\n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34592170\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/34592170\/<\/a><\/p>\n<p>To identify SARS-CoV-2 S-directed antibodies, authors utilized <strong>LIBRA-seq<\/strong>, a technology that enables high-throughput simultaneous determination of B cell receptor sequence and antigen reactivity at the single-cell level, expediting the process of lead candidate selection and characterization. Antigen-specific B cells were isolated from a donor with potently neutralizing antibodies in serum 3 months after infection confirmed by nasal swab RT-PCR testing for SARS-CoV-2. <\/p>\n<p>Of the 73 IgG+ B cells with high LIBRA-seq scores for SARS-CoV-2 Spike, nine lead candidates were selected for characterization as recombinant monoclonal antibodies.<br \/>\nFrom VSV assays, antibody named 54042-4 showed the best potency, at a IC<sub>50<\/sub> of 9 ng\/mL, and broad neutralization against existing SARS-CoV-2 variants.<br \/>\nIt was al so found that the 54042-4 heavy chain binds to RBD residues 443\u2013447 and the 54042-4 light chain contacts RBD residues 498\u2013500 from cryo-EM structure analysis.<\/p>\n<p><a href=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/10\/54042-4_antibody-_SARS-CoV-2.png\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/10\/54042-4_antibody-_SARS-CoV-2-300x164.png\" alt=\"\" width=\"300\" height=\"164\" class=\"alignnone size-medium wp-image-6319\" srcset=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/10\/54042-4_antibody-_SARS-CoV-2-300x164.png 300w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/10\/54042-4_antibody-_SARS-CoV-2-600x328.png 600w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/10\/54042-4_antibody-_SARS-CoV-2.png 721w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A group from Vanderbilt University Medical Center, Nashville, USA, etc. has reported that a highly potent and <\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/a-highly-potent-and-broad-neutralization-antibody-against-sars-cov-2-was-screened\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-6325","post","type-post","status-publish","format-standard","hentry","category-technology-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/6325","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=6325"}],"version-history":[{"count":2,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/6325\/revisions"}],"predecessor-version":[{"id":6345,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/6325\/revisions\/6345"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=6325"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=6325"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=6325"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}