{"id":5813,"date":"2021-08-18T10:00:14","date_gmt":"2021-08-18T01:00:14","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=5813"},"modified":"2021-08-22T08:27:21","modified_gmt":"2021-08-21T23:27:21","slug":"rhizosphere-of-maise-maize-root-lectins-mrls-mediate-interaction-with-herbaspirillum-seropedicae-via-galnac-of-lipopolysaccharides-lps","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/rhizosphere-of-maise-maize-root-lectins-mrls-mediate-interaction-with-herbaspirillum-seropedicae-via-galnac-of-lipopolysaccharides-lps\/","title":{"rendered":"Rhizosphere of Maise: Maize root lectins (MRLs) mediate interaction with Herbaspirillum seropedicae via O-GalNAc of Lipopolysaccharides (LPS)."},"content":{"rendered":"<p>A group from Universidade Federal do Paran\u00e1, Curitiba, Paran\u00e1, Brazil, etc. has reported that maize root lectins (MRLs) mediate interaction with Herbaspirillum seropedicae via O-GalNAc of Lipopolysaccharides (LPS).<br \/>\n<a href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0077001\">https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0077001<\/a><\/p>\n<p>The lipopolysaccharides (LPS) are highly complex macromolecules found exclusively as a monolayer on the outer membrane of gram-negative bacteria. These glycoconjugates are composed of three regions: the lipid A that anchors the molecule to the outer cell membrane, the core oligosaccharide, and the O-antigen. The LPS from Herbaspirillum seropedicae strain (wild type) has lipid A-core containing different degrees of O-antigen oligomerization, however, the LPS from the mutant strain LPSEB (waaL) lacks the O-antigen, has only the lipid A-core. Here, H. seropedicae is a plant growth-promoting diazotrophic betaproteobacterium which associates with important crops, such as maize, wheat, rice and sugar-cane.<\/p>\n<p>Maize root colonization of both wild type and mutant strains showed dose-dependent increases (as shown in a figure below A), however, the numbers of the waaL mutant cells attached to the roots were always lower than those of the wild type. Co-inoculation assays revealed a clear predominance of the wild type strain against the waaL mutant for attachment (see the figure below B).<\/p>\n<p>In the figure below, A: maize was inoculated separately with the indicated amount of each bacterial strain, and B: maize was inoculated with a 1\u22361 mixture of both strains. H. seropedicae wild-type is shown in black color and waaL mutant strain is shown in gray color.<br \/>\n<a href=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/08\/MRL_H.-seropedicae-wild-type-and-waaL-mutant-strains.png\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/08\/MRL_H.-seropedicae-wild-type-and-waaL-mutant-strains-208x300.png\" alt=\"\" width=\"208\" height=\"300\" class=\"alignnone size-medium wp-image-5792\" srcset=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/08\/MRL_H.-seropedicae-wild-type-and-waaL-mutant-strains-208x300.png 208w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/08\/MRL_H.-seropedicae-wild-type-and-waaL-mutant-strains.png 485w\" sizes=\"auto, (max-width: 208px) 100vw, 208px\" \/><\/a><\/p>\n<p>It is likely that the interaction between LPS O-antigen and the MRLs is a key step for the establishment of the bacteria onto the root enhancing H. seropedicae attachment and conferring a competitive advantage during colonization of root surfaces, to gain access to inner tissues.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A group from Universidade Federal do Paran\u00e1, Curitiba, Paran\u00e1, Brazil, etc. has reported that maize root lecti<\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/rhizosphere-of-maise-maize-root-lectins-mrls-mediate-interaction-with-herbaspirillum-seropedicae-via-galnac-of-lipopolysaccharides-lps\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70],"tags":[],"class_list":["post-5813","post","type-post","status-publish","format-standard","hentry","category-nature-en"],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO 4.9.8 - aioseo.com -->\n\t<meta name=\"description\" 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