{"id":5448,"date":"2021-08-01T06:30:26","date_gmt":"2021-07-31T21:30:26","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=5448"},"modified":"2021-07-30T11:51:55","modified_gmt":"2021-07-30T02:51:55","slug":"l-sign-is-a-receptor-for-authentic-sars-cov-2-virus-using-human-liver-sinusoidal-endothelial-cells-lsecs-with-no-ace2-but-l-sign","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/l-sign-is-a-receptor-for-authentic-sars-cov-2-virus-using-human-liver-sinusoidal-endothelial-cells-lsecs-with-no-ace2-but-l-sign\/","title":{"rendered":"L-SIGN is a receptor for authentic SARS-CoV-2 virus, using human liver sinusoidal endothelial cells (LSECs) with no ACE2 but L-SIGN"},"content":{"rendered":"

A group from Oklahoma Medical Research Foundation, Oklahoma, USA, etc. has reported that L-SIGN is a receptor for authentic SARS-CoV-2 infection.
\nhttps:\/\/insight.jci.org\/articles\/view\/148999<\/a><\/p>\n

To examine if L-SIGN interacts with SARS-CoV-2, lentivirus-based SARS-CoV-2 pseudo-typed virus (CoV-2\u2013type virus) and HEK293T cell lines expressing L-SIGN-flag or ACE2-myc3 as positive control were established. L-SIGN is a C-type lectin which binds to high-mannose\u2013type N-glycan and Ca2+ dependent manner. The RBD of the SARS-CoV-2 spike protein has an N-glycosylation site at Asn343. N-glycan\u2013deficient spike\/Fc, in which Asn343 was replaced with glutamine (Q) (SpikeN343Q\/Fc) was developed, and the mannose moiety deficiency on SpikeN343Q\/Fc were confirmed by mannose-specific GNL blotting. Thus, it was confirmed as shown below that SpikeN343Q\/Fc binds to cells expressing ACE2 but not to cells expressing L-SIGN, indicating that L-SIGN recognizes an N-glycan structure on Asn343. <\/p>\n

\"\"<\/a><\/p>\n

L-SIGN but not ACE2 is uniquely expressed on human liver sinusoidal endothelial cells (LSECs). To determine the clinical significance of the interaction between SARS-CoV-2 and L-SIGN, it was first examined if SARS-CoV-2 was present in LSECs from formalin-fixed paraffin-embedded liver autopsy samples from patients with COVID-19. In comparison with uninfected human liver autopsy samples, SARS-CoV-2 was detected with an anti-SARS-CoV-2 nucleocapsid antibody inside patient LSECs as shown below (LSECs were L-SIGN+ (green)and arrows mark SARS-CoV-2 (red)).<\/p>\n

\"\"<\/a><\/p>\n

In addition, it was shown that SARS-CoV-2 infection was blocked with an antibody against human L-SIGN and also with mannan dose-dependently, suggesting these could be potential therapeutic options to treat severe COVID-19 infection.<\/p>\n","protected":false},"excerpt":{"rendered":"

A group from Oklahoma Medical Research Foundation, Oklahoma, USA, etc. has reported that L-SIGN is a receptor <\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70],"tags":[],"class_list":["post-5448","post","type-post","status-publish","format-standard","hentry","category-nature-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/5448","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=5448"}],"version-history":[{"count":1,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/5448\/revisions"}],"predecessor-version":[{"id":5449,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/5448\/revisions\/5449"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=5448"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=5448"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=5448"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}