{"id":4896,"date":"2021-07-17T05:54:24","date_gmt":"2021-07-16T20:54:24","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=4877"},"modified":"2021-07-17T05:57:22","modified_gmt":"2021-07-16T20:57:22","slug":"a-bivalent-protein-targeting-oligo-mannose-in-sars-cov-2-spike-and-hr1-domain-in-s2-subunit-to-inhibit-sars-cov-2-infection","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/a-bivalent-protein-targeting-oligo-mannose-in-sars-cov-2-spike-and-hr1-domain-in-s2-subunit-to-inhibit-sars-cov-2-infection\/","title":{"rendered":"A bivalent protein targeting oligo-mannose in SARS-CoV-2 Spike and HR1 domain in S2 subunit to inhibit SARS-CoV-2 infection\u00a0"},"content":{"rendered":"

A group from Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, China, has developed a bivalent protein targeting oligo-mannose in SARS-CoV-2 Spike and HR1 domain in S2 subunit to inhibit SARS-CoV-2 infection.
\nhttps:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8264481\/<\/a><\/p>\n

To enter target cells, SARS-CoV-2 binds to its receptor ACE2 on the host cell through the receptor-binding domain (RBD) in S1 subunit of spike (S) protein. Such binding triggers conformation changes in the S2 subunit of S protein, resulting in the formation of a six-helix bundle (6-HB) between the heptad repeat 1 and 2 (HR1 and HR2) domains, thus bringing viral and target cell membranes together for fusion. Therefore, both S1 and S2 subunits can serve as important targets for the development of SARS-CoV-2 fusion and entry inhibitors. The sequence of S2 subunit is more conserved than that of S1 subunit, making it a better target for developing broad-spectrum CoV entry inhibitors.<\/p>\n

Authors aimed to design and construct a bivalent protein consisting of antiviral lectin GRFT and pan-CoV fusion inhibitory peptide EK1 and evaluate its inhibitory activity and mechanism of action against infection by SARS-CoV-2 and its mutants, as well as other human coronaviruses (HCoVs). Three types of recombinant plasmids encoding bivalent proteins (GRFT and EK1) with different linkers in the length, GRFT-L15-EK1 (GL15E), GRFT-L25-EK1 (GL25E), and GRFT-L35-EK1 (GL35E), containing linkers L15 (GGGGS)3<\/sub>, L25 (GGGGS)5<\/sub>, and L35 (GGGGS)7 <\/sub>between the GRFT and EK1 components.
\nIt was found that GL25E was the best to inhibit SARS-CoV-2 infection as shown below.<\/p>\n

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\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

A group from Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, China, has <\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70,7],"tags":[],"class_list":["post-4896","post","type-post","status-publish","format-standard","hentry","category-nature-en","category-technology-en"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t