{"id":4190,"date":"2021-06-15T10:20:47","date_gmt":"2021-06-15T01:20:47","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=4181"},"modified":"2021-06-15T10:34:21","modified_gmt":"2021-06-15T01:34:21","slug":"homer3-with-sialyl-t-and-sialyl-tn-antigen-could-be-a-therapeutic-target-for-bladder-cancer","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/homer3-with-sialyl-t-and-sialyl-tn-antigen-could-be-a-therapeutic-target-for-bladder-cancer\/","title":{"rendered":"HOMER3 with sialyl-T and sialyl-Tn antigen could be a therapeutic target for bladder cancer\u00a0"},"content":{"rendered":"

A group from Portuguese Institute of Oncology, etc. has reported that HOMER3 with sialyl-T and sialyl-Tn antigen could be a therapeutic target for bladder cancer (BC).
\nhttps:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8188679\/<\/a><\/p>\n

A total of 10 major glycans structures were identified for both BC cell lines: 5637 and T24, which present similar O-glycomes. Notably, both cell lines expressed high levels of mono- and di-sialylated T antigens (sialyl-T), also exhibiting neutral core 1 and sialylated and\/or fucosylated core 2 structures<\/p>\n

Based on glycomics analysis, samples were first enriched for membrane proteins by differential ultracentrifugation of whole cell protein extracts, digested with sialidase and enriched for T antigen expressing glycoproteins by PNA affinity pulldowns. The glycoproteins were identified by conventional bottom-up proteomics after trypsin digestion and analysis by nanoLC-CID-MS\/MS. As a result, over 900 glycoproteins were identified as potentially expressing sialyl-T.<\/p>\n

Among these, GLUT1(SLC2A1) and HOMER3 ranked first, suggesting potential for targeted therapeutics. GLUT1 is a pivotal membrane glucose transporter frequently overexpressed in more aggressive bladder tumours. HOMER3 has been implicated in neuronal signaling, T-cell activation and trafficking of beta amyloid peptides. HOMER3 at the cell membrane co-localizes in the same tumour area with sialyl-Tn and sialyl-T antigens in BC. According to immunohistochemistry, HOMER3 was diffusely expressed in wide tumour areas, both at the cytoplasm and cell membranes. These areas co-localized with very high sialyl-Tn and\/or sialyl-T expressions areas.<\/p>\n

\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

A group from Portuguese Institute of Oncology, etc. has reported that HOMER3 with sialyl-T and sialyl-Tn antig<\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70,7],"tags":[],"class_list":["post-4190","post","type-post","status-publish","format-standard","hentry","category-nature-en","category-technology-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/4190","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=4190"}],"version-history":[{"count":2,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/4190\/revisions"}],"predecessor-version":[{"id":4192,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/4190\/revisions\/4192"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=4190"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=4190"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=4190"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}