{"id":4163,"date":"2021-06-13T10:46:41","date_gmt":"2021-06-13T01:46:41","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=4150"},"modified":"2021-06-13T10:55:36","modified_gmt":"2021-06-13T01:55:36","slug":"aptamers-binding-to-both-the-glycan-and-the-near-the-peptide-region-of-human-prostate-specific-antigen-hpsa","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/aptamers-binding-to-both-the-glycan-and-the-near-the-peptide-region-of-human-prostate-specific-antigen-hpsa\/","title":{"rendered":"Aptamers binding to both the glycan and the near the peptide region of human prostate specific antigen (hPSA)\u00a0"},"content":{"rendered":"<p>Universidad de Oviedo, Spain has developed aptamers binding to both the glycan and the near the peptide region of human prostate specific antigen (hPSA).<br \/>\n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34094206\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/34094206\/<\/a><\/p>\n<p>To achieve a finer direction of the aptamers for integrating in the recognition both the glycan and the near peptide region of hPSA, Pholiota squarrosa lectin (PhoSL) was used as a specific lectin of \u03b11-6 core-fucose, increasing the likelihood of obtaining aptamers specific for the glycosylation site of the protein.<\/p>\n<p>The SELEX approach involved the immobilization of either hPSA or recombinant PSA (rPSA), which has no glycans, onto magnetic particles (MPs), using bovine serum albumin (BSA) as a blocking agent. Each SELEX round included a negative selection step with BSA\u2013MPs to eliminate sequences that bind to BSA, followed by a counter-selection against rPSA-MPs to remove molecules that bind to the protein by regions different from the glycosylation site, and a positive selection step with hPSA\u2013MPs to enrich the starting library in sequences that bind to the desired glycan moiety in the protein. <\/p>\n<p>After these initial rounds, the pool was split to perform two distinct routes. Strategy A includes an extra more stringent counter-selection step by blocking the \u03b11-6 core-fucose with PhoSL, removing molecules that remained bound to the blocked protein. Strategy B, in contrast, relies on the competitive elution of aptamers bound to the PhoSL binding site. Atrategy A leads to a decrease in the number of aptamers bound to hPSA, while the competitive elution with PhoSL produces a progressive increase in both population of hPSA-binders and their average binding affinity. <\/p>\n<p>A figure below shows binding specificity of selected aptamer (PSAG-1\uff09including two core-fucosylated glycoproteins: lipocalin-2 (NGAL) and \u03b1-fetoprotein (AFP). If more precise glycan structures specific to prostate caner could be recognized with this type of aptamers, it would be great.<\/p>\n<p><a href=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/06\/aptomer_PSAG-1.png\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/06\/aptomer_PSAG-1-300x262.png\" alt=\"\" width=\"300\" height=\"262\" class=\"alignleft size-medium wp-image-4152\" srcset=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/06\/aptomer_PSAG-1-300x262.png 300w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/06\/aptomer_PSAG-1.png 384w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Universidad de Oviedo, Spain has developed aptamers binding to both the glycan and the near the peptide region<\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/aptamers-binding-to-both-the-glycan-and-the-near-the-peptide-region-of-human-prostate-specific-antigen-hpsa\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-4163","post","type-post","status-publish","format-standard","hentry","category-technology-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/4163","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=4163"}],"version-history":[{"count":2,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/4163\/revisions"}],"predecessor-version":[{"id":4165,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/4163\/revisions\/4165"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=4163"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=4163"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=4163"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}