{"id":4102,"date":"2021-06-09T10:42:02","date_gmt":"2021-06-09T01:42:02","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=4097"},"modified":"2021-06-09T10:49:13","modified_gmt":"2021-06-09T01:49:13","slug":"human-surfactant-protein-d-would-have-therapeutic-potential-against-sars-cov-2-infection","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/human-surfactant-protein-d-would-have-therapeutic-potential-against-sars-cov-2-infection\/","title":{"rendered":"Human surfactant protein D would have therapeutic potential against SARS-CoV-2 infection\u00a0"},"content":{"rendered":"

A group from National Cheng Kung University, Tainan, etc. has reported that human surfactant protein D would have therapeutic potential against SARS-CoV-2 infection.
\nhttps:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8161545\/<\/a><\/p>\n

Human surfactant protein D (SP-D), a collagen-containing C-type lectin and a member of the collectin family, is known to be involved in pulmonary surfactant homeostasis and immunity. The ability of recombinant fragment of human SP-D(rfhSP-D) to inhibit infection of SARS-CoV-2 was examined using pseudotyped lentiviral particles expressing SARS-CoV-2 S1 protein.
\nIt was found that rfhSP-D bound SARS-CoV-2 S1 protein in a dose-dependent manner; this interaction was inhibited by maltose and EDTA. No rfhSP-D binding was observed in the absence of RBD in this assay.<\/p>\n

Inhibition of rfhSP-D binding to S protein by EDTA or maltose suggested that rfhSP-D bound to the carbohydrate moieties on S protein of SARS-CoV-2. A luciferase reporter assay with pseudotyped lentiviral particles expressing SARS-CoV-2 S1 protein was used to evaluate infectivity. Approximately 0.5 RLU fold reduction was seen with rfhSP-D (5 or 10 \u00b5g\/ml) treatment comparing to untreated sample (1 RLU fold; ACE2 overexpressing HEK293T cells + SARS-CoV-2). A significantly reduced luminescent signal following rfhSP-D treatment indicated that the interaction of rfhSP-D with SARS-CoV-2-S1 restricted the binding and entry of the virus.<\/p>\n

These results highlight the therapeutic potential of rfhSP-D in SARS-CoV-2 infection.\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"

A group from National Cheng Kung University, Tainan, etc. has reported that human surfactant protein D would h<\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70,7],"tags":[],"class_list":["post-4102","post","type-post","status-publish","format-standard","hentry","category-nature-en","category-technology-en"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t