{"id":4102,"date":"2021-06-09T10:42:02","date_gmt":"2021-06-09T01:42:02","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=4097"},"modified":"2021-06-09T10:49:13","modified_gmt":"2021-06-09T01:49:13","slug":"human-surfactant-protein-d-would-have-therapeutic-potential-against-sars-cov-2-infection","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/human-surfactant-protein-d-would-have-therapeutic-potential-against-sars-cov-2-infection\/","title":{"rendered":"Human surfactant protein D would have therapeutic potential against SARS-CoV-2 infection\u00a0"},"content":{"rendered":"<p>A group from National Cheng Kung University, Tainan, etc. has reported that human surfactant protein D would have therapeutic potential against SARS-CoV-2 infection.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8161545\/\">https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8161545\/<\/a><\/p>\n<p>Human surfactant protein D (SP-D), a collagen-containing C-type lectin and a member of the collectin family, is known to be involved in pulmonary surfactant homeostasis and immunity. The ability of recombinant fragment of human SP-D(rfhSP-D) to inhibit infection of SARS-CoV-2 was examined using pseudotyped lentiviral particles expressing SARS-CoV-2 S1 protein.<br \/>\nIt was found that rfhSP-D bound SARS-CoV-2 S1 protein in a dose-dependent manner; this interaction was inhibited by maltose and EDTA. No rfhSP-D binding was observed in the absence of RBD in this assay.<\/p>\n<p>Inhibition of rfhSP-D binding to S protein by EDTA or maltose suggested that rfhSP-D bound to the carbohydrate moieties on S protein of SARS-CoV-2. A luciferase reporter assay with pseudotyped lentiviral particles expressing SARS-CoV-2 S1 protein was used to evaluate infectivity. Approximately 0.5 RLU fold reduction was seen with rfhSP-D (5 or 10 \u00b5g\/ml) treatment comparing to untreated sample (1 RLU fold; ACE2 overexpressing HEK293T cells + SARS-CoV-2). A significantly reduced luminescent signal following rfhSP-D treatment indicated that the interaction of rfhSP-D with SARS-CoV-2-S1 restricted the binding and entry of the virus.<\/p>\n<p>These results highlight the therapeutic potential of rfhSP-D in SARS-CoV-2 infection.\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A group from National Cheng Kung University, Tainan, etc. has reported that human surfactant protein D would h<\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/human-surfactant-protein-d-would-have-therapeutic-potential-against-sars-cov-2-infection\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70,7],"tags":[],"class_list":["post-4102","post","type-post","status-publish","format-standard","hentry","category-nature-en","category-technology-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/4102","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=4102"}],"version-history":[{"count":3,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/4102\/revisions"}],"predecessor-version":[{"id":4105,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/4102\/revisions\/4105"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=4102"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=4102"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=4102"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}