{"id":4011,"date":"2021-06-01T16:20:10","date_gmt":"2021-06-01T07:20:10","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=3999"},"modified":"2021-06-01T16:34:12","modified_gmt":"2021-06-01T07:34:12","slug":"c-type-lectins-expressed-on-myeloid-cells-induce-proinflammatory-responses-through-the-interaction-with-sars-cov-2","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/c-type-lectins-expressed-on-myeloid-cells-induce-proinflammatory-responses-through-the-interaction-with-sars-cov-2\/","title":{"rendered":"C-type lectins expressed on myeloid cells induce proinflammatory responses through the interaction with SARS-CoV-2\u00a0"},"content":{"rendered":"<p>A group from New York University Grossman School of Medicine, etc. has reported that C-type lectins expressed on myeloid cells induce proinflammatory responses through the interaction with SARS-CoV-2.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8106883\/\">https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8106883\/<\/a><\/p>\n<p>It was investigated that the binding of human immunoglobulin Fc-tagged SARS-CoV-2 S, S1, and RBD recombinant proteins to HEK293T cells transfected with individual cDNA including five C-type lectins (DC-SIGN, L-SIGN, LSECtin, ASGR1, and CLEC10A) and Tweety family member 2 (TTYH2). It was clearly shown that the ectopic expression of the myeloid cell receptors in HEK293T cells supported SARS-CoV-2 engagement independently from ACE2. The binding of DC-SIGN, L-SIGN, ASGR1, and CLEC10A was not affected by soluble ACE2-His, but that of ACE2 and LSECtin was completely blocked by soluble ACE2-His, and also the binding of DC-SIGN and L-SIGN was completely inhibited by mannan.<\/p>\n<p><a href=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/06\/C-type-lectins_SARS-CoV-2.png\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/06\/C-type-lectins_SARS-CoV-2-300x231.png\" alt=\"\" width=\"300\" height=\"231\" class=\"alignleft size-medium wp-image-4001\" srcset=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/06\/C-type-lectins_SARS-CoV-2-300x231.png 300w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/06\/C-type-lectins_SARS-CoV-2-600x463.png 600w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/06\/C-type-lectins_SARS-CoV-2.png 686w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p>Furthermore, GFP-encoding HIV-based lentivirus pseudotyped with SARS-CoV-2 S protein (SARS-CoV-2 pseudovirus) was used to evaluate binding to those myeloid cell receptors. In the presence of the SARS-CoV-2 pseudovirus, robust GFP signals were observed in HEK293T expressing ectopic DC-SIGN or L-SIGN as well as expressing ectopic ACE2. The low level of SARS-CoV-2 pseudovirus engagement through LSECtin, ASGR1, CLEC10A, and TTYH2 was enhanced by co-expression of furin and\/or TMPRSS2, which mediate S protein cleavage for viral entry. Consistent with our S protein mannan blockade results, pseudovirus-derived GFP signals through DC-SIGN and L-SIGN were inhibited by mannan treatment. These data suggested that these myeloid cell receptors can engage with the SARS-CoV-2 S protein on the pseudotyped virus.<\/p>\n<p><a href=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/06\/SARS-CoV-2-pseudovirus_C-type-lectin.png\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/06\/SARS-CoV-2-pseudovirus_C-type-lectin-300x190.png\" alt=\"\" width=\"300\" height=\"190\" class=\"alignleft size-medium wp-image-4007\" srcset=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/06\/SARS-CoV-2-pseudovirus_C-type-lectin-300x190.png 300w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/06\/SARS-CoV-2-pseudovirus_C-type-lectin.png 482w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A group from New York University Grossman School of Medicine, etc. has reported that C-type lectins expressed <\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/c-type-lectins-expressed-on-myeloid-cells-induce-proinflammatory-responses-through-the-interaction-with-sars-cov-2\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70],"tags":[],"class_list":["post-4011","post","type-post","status-publish","format-standard","hentry","category-nature-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/4011","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=4011"}],"version-history":[{"count":2,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/4011\/revisions"}],"predecessor-version":[{"id":4013,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/4011\/revisions\/4013"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=4011"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=4011"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=4011"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}