{"id":3991,"date":"2021-06-01T06:43:19","date_gmt":"2021-05-31T21:43:19","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=3976"},"modified":"2021-06-01T06:47:32","modified_gmt":"2021-05-31T21:47:32","slug":"unbelievable-discovery-that-rna-is-glycosylated","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/unbelievable-discovery-that-rna-is-glycosylated\/","title":{"rendered":"Unbelievable discovery that RNA is glycosylated\u00a0"},"content":{"rendered":"<p>A group from Stanford University, etc. has reported that small noncoding RNAs are the third scaffold of glycosylation.<br \/>\n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34004145\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/34004145\/<\/a><\/p>\n<p>Existence of glycosylated RNAs (named GlycoRNAs) was confirmed with the following protocol. Authors metabolically labeled cells with peracetylated N-azidoacetylmannosamine (Ac4ManNAz), and RNA was extracted with warm TRIzol, then ethanol precipitated, desalted via silica columns, stripped of protein contamination via high concentration proteinase K digestion, and repurified over silica columns. After RNA purification, Ac4ManNAz was conjugated to DBCO-biotin, visualized with streptavidin-IR800 (Strep), and imaged on an infrared scanner. <\/p>\n<p>The RNA scaffolds for glycosylation was found to be small noncoding ones. Most of glycoRNAs existed on cell surface, and GlycoRNAs were modified with complex-type N-glycans with at least one terminal sialic acid residue. It was found that these GlycoRNAs directly bind to Siglecs.<\/p>\n<p><a href=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/05\/GlycoRNA_Siglec.png\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/05\/GlycoRNA_Siglec-233x300.png\" alt=\"\" width=\"233\" height=\"300\" class=\"alignleft size-medium wp-image-3981\" srcset=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/05\/GlycoRNA_Siglec-233x300.png 233w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/05\/GlycoRNA_Siglec.png 279w\" sizes=\"auto, (max-width: 233px) 100vw, 233px\" \/><\/a><\/p>\n<p>The framework in which glycobiology is presently understood excludes RNA as a substrate for N-glycosylation. This discovery of glycoRNA suggests the current view is incomplete and points to a new axis of RNA glycobiology, including as of yet undiscovered biosynthetic and trafficking mechanisms. Further, it highlights the possibility that cell surface glycoconjugates, which mediate and regulate important inter-cellular interactions.\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A group from Stanford University, etc. has reported that small noncoding RNAs are the third scaffold of glycos<\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/unbelievable-discovery-that-rna-is-glycosylated\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70],"tags":[],"class_list":["post-3991","post","type-post","status-publish","format-standard","hentry","category-nature-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/3991","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=3991"}],"version-history":[{"count":2,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/3991\/revisions"}],"predecessor-version":[{"id":3993,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/3991\/revisions\/3993"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=3991"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=3991"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=3991"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}