{"id":3934,"date":"2021-05-24T12:25:30","date_gmt":"2021-05-24T03:25:30","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=3926"},"modified":"2021-05-24T12:29:58","modified_gmt":"2021-05-24T03:29:58","slug":"an-infection-mechanism-of-sars-cov-2-infection-through-c-type-lectin-receptors-clrs","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/an-infection-mechanism-of-sars-cov-2-infection-through-c-type-lectin-receptors-clrs\/","title":{"rendered":"An infection mechanism of SARS-CoV-2 infection through C-type lectin receptors (CLRs)\u00a0"},"content":{"rendered":"

A group from Univ. Grenoble Alpes, France, etc. has reported a mechanism of SARS-CoV-2 infection through C-type lectin receptors (CLRs).
\nhttps:\/\/journals.plos.org\/plospathogens\/article\/authors?id=10.1371\/journal.ppat.1009576<\/a><\/p>\n

Authors studies molecular interaction between various CLRs (DC-SIGN, L-SIGN, MGL, and Langerin) with SARS-CoV-2 spike protein using SPR. DC-SIGN, L-SIGN, and MGL showed affinities in the \u03bcMrange in terms of Kd, from around 2 to 10 \u03bcM, and that of Langerin was speculated to be weaker at least one order of magnitude.<\/p>\n

In order to understand the roles of CLRs in SARS-CoV-2 infection, infection experiments were performed using monocyte-derived dendritic cells (MDDCs), monocyte-derived macrophages (M2-MDM) and Vero E6 cells. Interestingly, VSV\/SARS-CoV-2 pseudotyped virus did not infect MDDCs or M2-MDMs, despite DC-SIGN expression. In the case of VSV\/EBOV-GP, however, direct infection was observed and DC-SIGN-mediated cis-infection was clearly blocked with anti-DC\/L-SIGN in the case of MDDCs (92.5% inhibition of infection), followed by M2-MDM (68.4% inhibition). VSV\/VSV-G also showed strong infectivity to both MDDC and M2-MDM. However, this infection was DC-SIGN independent, since anti-DC-SIGN antibodies did not impact the infection level.<\/p>\n

To study the potential function of DC\/L-SIGN in SARS-CoV-2 trans-infection, MDDCs were incubated with VSV\/SARS-CoV-2 and placed onto susceptible Vero E6 cells which is the reference ACE2+ cell line for SARS-CoV-2 cell culture. Interestingly, DC-SIGN promoted efficient SARS-CoV-2 transfer from MDDC to Vero E6. An anti-DC-SIGN antibody could reduce substantially the infectivity observed (98% inhibition). These experiments confirmed that the role of DC-SIGN and L-SIGN in the process of SARS-CoV-2 could be trans-infection.<\/p>\n

\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

A group from Univ. Grenoble Alpes, France, etc. has reported a mechanism of SARS-CoV-2 infection through C-typ<\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70],"tags":[],"class_list":["post-3934","post","type-post","status-publish","format-standard","hentry","category-nature-en"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t