{"id":3844,"date":"2021-05-20T08:35:54","date_gmt":"2021-05-19T23:35:54","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=3835"},"modified":"2021-05-20T08:43:22","modified_gmt":"2021-05-19T23:43:22","slug":"poct-biosensor-named-rapid-for-sars-cov-2-based-on-electrochemical-reading-with-quite-high-sensitivity-and-high-detection-speed","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/poct-biosensor-named-rapid-for-sars-cov-2-based-on-electrochemical-reading-with-quite-high-sensitivity-and-high-detection-speed\/","title":{"rendered":"POCT biosensor (named RAPID) for SARS-CoV-2 based on electrochemical reading with quite high sensitivity and high detection speed"},"content":{"rendered":"

A group from Perelman School of Medicine, University of Pennsylvania, etc. has reported a POCT biosensor (named RAPID) for SARS-CoV-2 based on electrochemical reading with quite high sensitivity and high detection speed.
\nhttps:\/\/pubmed.ncbi.nlm.nih.gov\/33997767\/<\/a><\/p>\n

ACE2 was used as a probe detecting SARS-CoV-2 Spike protein. Enzyme immobilization on the electrode was done by cross-linking ACE2 using the bifunctional chemical cross-linker glutaraldehyde. BSA was used to block the electrode\u2019s surface after immobilization of ACE2. Nafion was added to increase the sensitivity further.
\nThe binding between two molecules (ACE2 and SARS-CoV-2 Spike protein) causes a change in interfacial electron transfer kinetics between the redox probe, ferricyanide\/ferrocyanide in solution and the conducting electrode sites. This electrochemical change is then detectable by monitoring the charge-transfer resistance.<\/p>\n

As a result, a linear concentration range from 10 fg\/mL to 100 ng\/mL was obtained (R2 = 0.993) and limits of detection (LOD) and quantification (LOQ) were calculated as 2.18 fg\/mL on signal to noise ratios (S\/N=3). With RAPID, result is obtained in 4 minutes (2 minutes of sample incubation + 2 minutes to perform the EIS analysis)..<\/p>\n

In blinded tests using 139 nasopharyngeal swab samples, 109 of which were COVID-19 positive and 30 COVID-19 negative as determined by RT-qPCR, RAPID demonstrated high sensitivity, specificity and accuracy for nasopharyngeal (83.5%, 100% and 87.1%, respectively) and saliva (100%, 86.5% and 90.0%, respectively) samples.<\/p>\n

\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

A group from Perelman School of Medicine, University of Pennsylvania, etc. has reported a POCT biosensor (name<\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-3844","post","type-post","status-publish","format-standard","hentry","category-technology-en"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t