{"id":3443,"date":"2021-04-26T16:43:38","date_gmt":"2021-04-26T07:43:38","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=3431"},"modified":"2021-04-27T16:44:49","modified_gmt":"2021-04-27T07:44:49","slug":"in-order-to-improve-productivity-of-mabs-with-cho-controlling-those-glycan-structures","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/in-order-to-improve-productivity-of-mabs-with-cho-controlling-those-glycan-structures\/","title":{"rendered":"In order to improve productivity of mAbs with CHO controlling those glycan structures\u00a0"},"content":{"rendered":"<p>A group from Daiichi Sankyo Co., Ltd. has reported about a new chemical which is added into CHO culture media and thereby is able to improve productivity of mAbs as therapeutic drugs.<br \/>\n<a href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0250416\">https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0250416<\/a><\/p>\n<p>Authors intended to improve productivity of mAbs with controlling those glycan modification by adding a chemical into CHO culture media.<br \/>\nThey started screening from 23,277 chemicals, and through the following 2nd screening condition; over 120% for relative mAb concentration, 105% for relative cell-specific productivity, and 80% for viability, a few candidates were selected. From the final selectins, 4-(2,5-dimethyl-1H-pyrrol-1-yl)-N-(2,5-dioxopyrrolidin-1-yl) benzamide (MPPB) was selected as a most suitable chemical to meet the intended requirement. MPPB concentrations of 0.32 to 0.64 mM were used, and with this addition, the productivity of mAbs increased by 1.5times.<br \/>\nG0F was the major N-linked glycan, and G1F was decreased from 24.5 to 14.8% under the MPPB-added condition, although others were not changed<\/p>\n<p><a href=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/04\/N-glycan-ratio_CHO.png\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/04\/N-glycan-ratio_CHO-300x180.png\" alt=\"\" width=\"300\" height=\"180\" class=\"alignleft size-medium wp-image-3440\" srcset=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/04\/N-glycan-ratio_CHO-300x180.png 300w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/04\/N-glycan-ratio_CHO-600x361.png 600w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/04\/N-glycan-ratio_CHO.png 750w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A group from Daiichi Sankyo Co., Ltd. has reported about a new chemical which is added into CHO culture media <\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/in-order-to-improve-productivity-of-mabs-with-cho-controlling-those-glycan-structures\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-3443","post","type-post","status-publish","format-standard","hentry","category-technology-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/3443","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=3443"}],"version-history":[{"count":5,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/3443\/revisions"}],"predecessor-version":[{"id":3454,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/3443\/revisions\/3454"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=3443"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=3443"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=3443"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}