{"id":2253,"date":"2021-02-11T11:18:53","date_gmt":"2021-02-11T02:18:53","guid":{"rendered":"http:\/\/www.emukk.com\/WP\/?p=2230"},"modified":"2021-02-11T12:11:30","modified_gmt":"2021-02-11T03:11:30","slug":"electrochemical-high-sensitivity-detection-using-rca-amplification-and-redox-reaction-of-the-novel-coronavirus-sars-cov-2","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/electrochemical-high-sensitivity-detection-using-rca-amplification-and-redox-reaction-of-the-novel-coronavirus-sars-cov-2\/","title":{"rendered":"Electrochemical high sensitivity detection using RCA isothermal amplification and Redox reaction of the novel coronavirus (SARS-CoV-2)"},"content":{"rendered":"

RT-PCR is the Gold Standard for the detection of the new coronavirus (SARS-CoV-2).
\nA group from Mahidol University, Nakhon Pathom, Thailand etc. has reported an electrochemical detection method that has the same sensitivity and specificity as RT-PCR and do not require expensive devices.
\nhttps:\/\/www.nature.com\/articles\/s41467-021-21121-7<\/a><\/p>\n

In order to detect SARS-CoV-2 with high sensitivity, of course, a gene amplification process is required. They adopted Rolling Circle Amplification (RCA). The RCA method, like the PCR and LAMP methods, has the advantage that carryover contamination has a small impact on the next amplification process and can be amplified in an isothermal bath at around room temperature (25\u00b0C to 37\u00b0C). The RCA amplicon (Padlock probe) was designed as shown below and was consist of Target gene specific to the target you want to detect (N-gene, S-gene, etc. of SARS-CoV-2), Universal capture probe-binding region, and Generic reporter probe-binding region. With this Padlock probe, RCA results in long DNA amplicons containing hundreds of tandem repeats of the Padlock DNA complementary sequence. For this RCA products, hybridize probe-conjugated magnetic bead particle (CP-MNB) and Silica methylene blue reporter probe (SiMB-RP) to obtain the final product. Methylene blue or Acridine orange was used to as a redox regent. The current obtained by the electrochemical reaction increased with copy numbers as shown below. As a result, the detection limit sensitivity reached 1 copy\/uL.<\/p>\n

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RT-PCR is the Gold Standard for the detection of the new coronavirus (SARS-CoV-2). A group from Mahidol Univer<\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-2253","post","type-post","status-publish","format-standard","hentry","category-technology-en"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t