{"id":2230,"date":"2021-02-11T11:18:53","date_gmt":"2021-02-11T02:18:53","guid":{"rendered":"http:\/\/www.emukk.com\/WP\/?p=2230"},"modified":"2021-02-11T16:10:07","modified_gmt":"2021-02-11T07:10:07","slug":"%e6%96%b0%e5%9e%8b%e3%82%b3%e3%83%ad%e3%83%8a%e3%82%a6%e3%82%a4%e3%83%ab%e3%82%b9%ef%bc%88sars-cov-2%ef%bc%89%e3%81%aerca%e5%a2%97%e5%b9%85%e3%81%a8redox%e5%8f%8d%e5%bf%9c%e3%82%92%e7%94%a8%e3%81%84","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/%e6%96%b0%e5%9e%8b%e3%82%b3%e3%83%ad%e3%83%8a%e3%82%a6%e3%82%a4%e3%83%ab%e3%82%b9%ef%bc%88sars-cov-2%ef%bc%89%e3%81%aerca%e5%a2%97%e5%b9%85%e3%81%a8redox%e5%8f%8d%e5%bf%9c%e3%82%92%e7%94%a8%e3%81%84\/","title":{"rendered":"\u65b0\u578b\u30b3\u30ed\u30ca\u30a6\u30a4\u30eb\u30b9\uff08SARS-CoV-2\uff09\u306eRCA\u5897\u5e45\u3068Redox\u53cd\u5fdc\u3092\u7528\u3044\u305f\u96fb\u6c17\u5316\u5b66\u7684\u306a\u9ad8\u611f\u5ea6\u691c\u51fa\u6cd5"},"content":{"rendered":"

\u65b0\u578b\u30b3\u30ed\u30ca\u30a6\u30a4\u30eb\u30b9\uff08SARS-CoV-2\uff09\u691c\u51fa\u306eGold Standard\u3068\u8a00\u3048\u3070\u3001RT-PCR\u3067\u3042\u308a\u307e\u3059\u3002
\nMahidol University, Nakhon Pathom, Thailand\u3089\u306e\u30b0\u30eb\u30fc\u30d7\u306f\u3001RT-PCR\u3068\u540c\u30ec\u30d9\u30eb\u306e\u611f\u5ea6\u3068\u7279\u7570\u5ea6\u3092\u6709\u3057\u3001\u304b\u3064\u9ad8\u984d\u306a\u88c5\u7f6e\u3082\u5fc5\u8981\u3068\u3057\u306a\u3044\u96fb\u6c17\u5316\u5b66\u7684\u306a\u691c\u51fa\u6cd5\u306b\u3064\u3044\u3066\u5831\u544a\u3057\u3066\u3044\u307e\u3059\u3002
\nhttps:\/\/www.nature.com\/articles\/s41467-021-21121-7<\/a><\/p>\n

SARS-CoV-2\u3092\u9ad8\u611f\u5ea6\u306b\u691c\u51fa\u3059\u308b\u306b\u306f\u3001\u5f53\u7136\u306a\u304c\u3089\u907a\u4f1d\u5b50\u306e\u5897\u5e45\u5de5\u7a0b\u304c\u5fc5\u8981\u3067\u3059\u3002\u7b46\u8005\u3089\u306f\u3001Rolling Circle Amplification\uff08RCA\uff09\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002RCA\u6cd5\u306f\u3001PCR\u6cd5\u3084LAMP\u6cd5\u3068\u306f\u7570\u306a\u308a\u3001\u30ad\u30e3\u30ea\u30fc\u30aa\u30fc\u30d0\u30fc\u30b3\u30f3\u30bf\u30df\u30cd\u30fc\u30b7\u30e7\u30f3\u3092\u8d77\u3053\u3057\u3066\u3082\u6b21\u306e\u5897\u5e45\u5de5\u7a0b\u306b\u4e0e\u3048\u308b\u5f71\u97ff\u304c\u5c0f\u3055\u3044\u3001\u5ba4\u6e29\u7a0b\u5ea6\uff0825\u2103\uff5e37\u2103\uff09\u306e\u7b49\u6e29\u5de5\u7a0b\u3067\u5897\u5e45\u3067\u304d\u308b\u3001\u3068\u3044\u3063\u305f\u5229\u70b9\u3092\u6301\u3063\u3066\u3044\u307e\u3059\u3002RCA amplicon (Padlock probe)\u306f\u3001\u4e0b\u56f3\u306e\u3088\u3046\u306b\u8a2d\u8a08\u3055\u308c\u3001\u691c\u51fa\u3057\u305f\u3044\u30bf\u30fc\u30b2\u30c3\u30c8(SARS-CoV-2\u306eN-gene, S-gene\u306a\u3069)\u306b\u7279\u7570\u7684\u306a\u914d\u5217(Target gene), Universal capture probe-binding region, Gene specific reporter probe-binding region\u304b\u3089\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u3053\u306ePadlock probe\u3092\u7528\u3044\u3066RCA\u3092\u884c\u3046\u3068\u3001Target gene\u3089\u304c\u7e70\u308a\u8fd4\u3057\u73fe\u308c\u308b\u5de8\u5927\u306a\u9577\u9396\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u3053\u306eRCA products\u306b\u5bfe\u3057\u3066\u3001probe-conjugated magnetic bead particle (CP-MNB),\u00a0Silica methylene blue reporter probe (SiMB-RP)\u3092\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30ba\u3057\u3066\u6700\u7d42\u7684\u306a\u7523\u7269\u3092\u5f97\u307e\u3059\u3002Redox\u53cd\u5fdc\u3092\u8d77\u3053\u3055\u305b\u308b\u305f\u3081\u306bMethylene blue\u3084Acridine orange\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u96fb\u6c17\u5316\u5b66\u53cd\u5fdc\u306b\u3088\u3063\u3066\u5f97\u3089\u308c\u308b\u96fb\u6d41\u306f\u3001\u4e0b\u56f3\u306e\u3088\u3046\u306bcopy number\u3068\u5171\u306b\u5897\u52a0\u3057\u307e\u3059\u3002\u7d50\u679c\u3068\u3057\u3066\u3001\u691c\u51fa\u9650\u754c\u611f\u5ea6\u306f\u30011 copy\/uL\u304c\u5f97\u3089\u308c\u305f\u3068\u306e\u3053\u3068\u3067\u3059\u3002<\/p>\n

\"\"<\/a><\/p>\n

\"\"<\/a><\/p>\n

\"\"<\/a><\/p>\n

\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u65b0\u578b\u30b3\u30ed\u30ca\u30a6\u30a4\u30eb\u30b9\uff08SARS-CoV-2\uff09\u691c\u51fa\u306eGold Standard\u3068\u8a00\u3048\u3070\u3001RT-PCR\u3067\u3042\u308a\u307e\u3059\u3002 Mahidol University, Nakhon Pathom, Thailand\u3089\u306e\u30b0\u30eb\u30fc\u30d7\u306f\u3001RT-P<\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42],"tags":[],"class_list":["post-2230","post","type-post","status-publish","format-standard","hentry","category-technology-ja"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/2230","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=2230"}],"version-history":[{"count":12,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/2230\/revisions"}],"predecessor-version":[{"id":2263,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/2230\/revisions\/2263"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=2230"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=2230"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=2230"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}