{"id":1971,"date":"2021-01-23T16:49:21","date_gmt":"2021-01-23T07:49:21","guid":{"rendered":"http:\/\/www.emukk.com\/WP\/?p=1971"},"modified":"2021-01-23T17:21:19","modified_gmt":"2021-01-23T08:21:19","slug":"%e3%82%a2%e3%83%b3%e3%83%a2%e3%83%8b%e3%82%a2%e3%82%92%e4%bd%bf%e3%81%a3%e3%81%a6%e5%a4%9a%e8%83%bd%e6%80%a7%e5%b9%b9%e7%b4%b0%e8%83%9e%ef%bc%88ips-es%ef%bc%89%e3%82%88%e3%82%8a%e5%88%86%e5%8c%96","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/%e3%82%a2%e3%83%b3%e3%83%a2%e3%83%8b%e3%82%a2%e3%82%92%e4%bd%bf%e3%81%a3%e3%81%a6%e5%a4%9a%e8%83%bd%e6%80%a7%e5%b9%b9%e7%b4%b0%e8%83%9e%ef%bc%88ips-es%ef%bc%89%e3%82%88%e3%82%8a%e5%88%86%e5%8c%96\/","title":{"rendered":"\u30a2\u30f3\u30e2\u30cb\u30a2\u3092\u4f7f\u3063\u3066\u591a\u80fd\u6027\u5e79\u7d30\u80de\uff08iPSC, ESC\uff09\u3088\u308a\u5206\u5316\u3055\u305b\u305f\u809d\u7d30\u80de\u3092\u9078\u5225\u3059\u308b\u65b9\u6cd5"},"content":{"rendered":"<p>\u809d\u7d30\u80de\u3092\u7528\u3044\u305f\u6bd2\u6027\u8a66\u9a13\u306f\u3001\u85ac\u7269\u306e\u6bd2\u6027\u8a66\u9a13\u3092\u52d5\u7269\u5b9f\u9a13\u3067\u884c\u3046\u65b9\u6cd5\u306e\u4ee3\u66ff\u6280\u8853\u3068\u3057\u3066\u5e83\u304f\u4f7f\u7528\u3055\u308c\u308b\u306b\u81f3\u3063\u3066\u3044\u307e\u3059\u3002\u6bd2\u6027\u8a66\u9a13\u3067\u809d\u7d30\u80de\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u306e\u554f\u984c\u70b9\u306f\u3001\u540c\u3058\u7279\u6027\u3092\u6301\u3064\u809d\u7d30\u80de\u3092\u5927\u91cf\u306b\u5b89\u5b9a\u4f9b\u7d66\u3067\u304d\u306a\u3044\u3068\u3044\u3046\u3068\u3053\u308d\u306b\u3042\u308a\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u56fd\u7acb\u6210\u80b2\u533b\u7642\u7814\u7a76\u6240\u306e\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u591a\u80fd\u6027\u7d30\u80de\uff08iPSC, ESC\uff09\u304b\u3089\u809d\u7d30\u80de\u3092\u4f5c\u308a\u3001\u30a2\u30f3\u30e2\u30cb\u30a2\u3092\u4f7f\u3063\u3066\u4e0d\u5747\u4e00\u306a\u7d30\u80de\u96c6\u56e3\u304b\u3089\u5747\u4e00\u6027\u306e\u9ad8\u3044\u809d\u7d30\u80de\u96c6\u56e3\u3092\u30a8\u30f3\u30ea\u30c3\u30c1\u3057\u3066\u304f\u308b\u65b9\u6cd5\u3092\u63d0\u6848\u3057\u3066\u307e\u3059\u3002<br \/>\n<a href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2020.03.20.999680v1.full\">https:\/\/www.biorxiv.org\/content\/10.1101\/2020.03.20.999680v1.full<\/a><\/p>\n<p>ESC\u3092\u809d\u7d30\u80de\u306b\u5206\u5316\u3055\u305b\u305f\u5f8c\u30012\u65e5\u9593\u306e\u30a2\u30f3\u30e2\u30cb\u30a2\u51e6\u7406\u3092\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u30a2\u30f3\u30e2\u30cb\u30a2\u6bd2\u6027\u306b\u8010\u6027\u3092\u6301\u3064\u5747\u4e00\u306a\u809d\u7d30\u80de\u3092\u9078\u5225\u3057\u307e\u3059\u300270\uff5e80%\u304c\u3053\u306e\u6bb5\u968e\u3067\u6b7b\u6ec5\u3057\u307e\u3059\u3002\u751f\u304d\u6b8b\u3063\u305f\u809d\u7d30\u80de\u3092MEF-feeder\u306e\u4e0a\u3067\u57f9\u990a\u3057\u3001\u5897\u6b96\u3055\u305b\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u3057\u3066\u9078\u5225\u3055\u308c\u305f\u809d\u7d30\u80de\u306f\u3001190\u65e5\u9593\u306730\u500d\u307b\u3069\u306b\u5897\u6b96\u3057\u307e\u3059\u3002\u30a2\u30f3\u30e2\u30cb\u30a2\u6bd2\u6027\u306e\u8a73\u7d30\u306a\u30e1\u30ab\u30cb\u30ba\u30e0\u306f\u5206\u304b\u3063\u3066\u3044\u307e\u305b\u3093\u304c\u3001\u30a2\u30f3\u30e2\u30cb\u30a2\u30a4\u30aa\u30f3\u304c\u30ab\u30ea\u30a6\u30e0\u30a4\u30aa\u30f3\u3068\u7af6\u5408\u3057\u3001\u6700\u7d42\u7684\u306b\u7d30\u80de\u819c\u5185\u5916\u306epH\u5909\u5316\u3067\u6b7b\u306b\u81f3\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u30a2\u30f3\u30e2\u30cb\u30a2\u9078\u5225\u3055\u308c\u305f\u809d\u7d30\u80de\u306f\u3001ALB, AFP, CYP3A4, CPS1, and OTC\u3089\u306e\u907a\u4f1d\u5b50\u304c\u9ad8\u767a\u73fe\u3057\u3066\u304a\u308a\u3001ALB\u3068AFP\u306f\u57f9\u990a\u65e5\u6570\u3068\u3068\u3082\u306b\u6e1b\u5c11\u3057\u3066\u3044\u304d\u307e\u3059\u3002CPS1, OTC\u306f\u3001\u30a2\u30f3\u30e2\u30cb\u30a2\u306e\u4ee3\u8b1d\u306b\u304b\u304b\u308f\u308b\u907a\u4f1d\u5b50\u3067\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u3001CYP3A4\u306f\u3001\u4e0d\u8981\u306a\u751f\u4f53\u7570\u7269\u3092\u4ee3\u8b1d\u3059\u308b\u9175\u7d20\u306e\u4e00\u3064\u3067\u3059\u3002<br \/>\n\u540c\u69d8\u306a\u3053\u3068\u306f\u3001iPSC\u304b\u3089\u4f5c\u3063\u305f\u809d\u7d30\u80de\u3067\u3082\u8a00\u3048\u307e\u3059\u3002<\/p>\n<p>iPSC, ESC\u3088\u308a\u5206\u5316\u3055\u305b\u305f\u7d30\u80de\u306f\u3001ESTEM-HE \u57f9\u5730 (GlycoTechnica, Ltd) 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