{"id":1744,"date":"2021-01-08T16:30:42","date_gmt":"2021-01-08T07:30:42","guid":{"rendered":"http:\/\/www.emukk.com\/WP\/?p=1740"},"modified":"2021-01-12T13:57:44","modified_gmt":"2021-01-12T04:57:44","slug":"limited-effect-of-glycans-on-the-binding-of-the-new-coronavirus-sars-cov-2-and-ace2","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/limited-effect-of-glycans-on-the-binding-of-the-new-coronavirus-sars-cov-2-and-ace2\/","title":{"rendered":"Limited effect of glycans on the binding of the new coronavirus (SARS-CoV-2) and ACE2"},"content":{"rendered":"<p>A group of Univ. of Southampton has investigated the effects of ACE2 glycan modification on the binding of S-protein of the new coronavirus (SARS-CoV-2) onto its infection receptor ACE2 .<br \/>\n<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0022283620306872?via%3Dihub\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0022283620306872?via%3Dihub<\/a><\/p>\n<p>By adding ST6 to WT ACE2 to increase sialic acid,<br \/>\nBy adding Sialidase to WT ACE2 to cleave sialic acid,<br \/>\nBy adding Kinfunensine to WT ACE2 to modify the glycan structure to Man9GlcNAc2 mainly,<br \/>\nBy adding EndoH to WT ACE2 to remove N-type sugar chains,<br \/>\nBy adding Fucosidase to WT ACE to remove Fuc,<br \/>\nand the binding of S-protein to ACE2 (Kd value) was measured by SPR.<\/p>\n<p><a href=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/01\/ace2_glycan-modification.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-full wp-image-1741\" src=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2021\/01\/ace2_glycan-modification.png\" alt=\"\" width=\"238\" height=\"211\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>When the N-glycans are removed from ACE2, the binding force is slightly increased, but the effect is limited.<br \/>\nFuc modification of ACE2 has little effect.<br \/>\nSialic acid modification of ACE2 does not seem to affect the infectivity, rather weaken the infectivity.<br \/>\nHigh mannose modification on ACE2 seems to weaken the infectivity slightly.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A group of Univ. of Southampton has investigated the effects of ACE2 glycan modification on the binding of S-p<\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/limited-effect-of-glycans-on-the-binding-of-the-new-coronavirus-sars-cov-2-and-ace2\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70],"tags":[],"class_list":["post-1744","post","type-post","status-publish","format-standard","hentry","category-nature-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/1744","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=1744"}],"version-history":[{"count":4,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/1744\/revisions"}],"predecessor-version":[{"id":1771,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/1744\/revisions\/1771"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=1744"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=1744"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=1744"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}