{"id":15000,"date":"2024-10-23T08:22:20","date_gmt":"2024-10-22T23:22:20","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=15000"},"modified":"2024-10-23T08:23:58","modified_gmt":"2024-10-22T23:23:58","slug":"lectin-staining-is-significantly-different-between-formalin-fixed-tissue-samples-and-frozen-tissue-samples","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/lectin-staining-is-significantly-different-between-formalin-fixed-tissue-samples-and-frozen-tissue-samples\/","title":{"rendered":"Lectin staining is significantly different between formalin-fixed tissue samples and frozen tissue samples"},"content":{"rendered":"<p>A group from Department of Gastroenterological surgery, Gifu University School of Medicine, Gifu, Japan, has reported about differences in glycocalyx morphology and composition in frozen and formalin-fixed liver tumor sections.<br \/>\n<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0344033824005715?via%3Dihub\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0344033824005715?via%3Dihub<\/a><\/p>\n<p>It was found that surprisingly, there were many differences in lectin staining findings between frozen and formali-fixed tissue preparations, suggesting that FFPE processing affects lectin receptors, rendering frozen sections more reliable for accurate lectin staining.<\/p>\n<p>From the lectin staining in frozen sections, the followings were found:<br \/>\nNormal hepatocytes showed strongly positive staining for PNA, RCA I, SBA, UEA I, GSL I, succinylated WGA, ECL, GSL II, STL, and VVL.<br \/>\nIn contrast, hepatocellular carcinoma samples were strongly positive for DSL and GSL II.<br \/>\nNormal hepatocytes were positive for multiple GalNAc-related lectins (PNA, SBA, GSL I, and VVL), but these were not detected in hepatocellular carcinoma samples.<\/p>\n<p>It was also found that DBA and UEA I staining were strongly positive in liver metastases from colorectal cancer and in melanoma liver metastases, ConA, WGA, succinylated WGA, and GSL II were strongly expressed.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A group from Department of Gastroenterological surgery, Gifu University School of Medicine, Gifu, Japan, has r<\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/lectin-staining-is-significantly-different-between-formalin-fixed-tissue-samples-and-frozen-tissue-samples\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70,7],"tags":[],"class_list":["post-15000","post","type-post","status-publish","format-standard","hentry","category-nature-en","category-technology-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/15000","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=15000"}],"version-history":[{"count":1,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/15000\/revisions"}],"predecessor-version":[{"id":15001,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/15000\/revisions\/15001"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=15000"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=15000"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=15000"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}