{"id":13616,"date":"2023-09-27T07:22:00","date_gmt":"2023-09-26T22:22:00","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=13616"},"modified":"2023-09-27T07:24:13","modified_gmt":"2023-09-26T22:24:13","slug":"an-antibody-for-6-sulfo-sialyl-lewis-x-glycan","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/an-antibody-for-6-sulfo-sialyl-lewis-x-glycan\/","title":{"rendered":"An antibody for 6-sulfo sialyl Lewis x glycan"},"content":{"rendered":"

A group from Laboratory of Microbiology and Immunology, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, Japan, etc. has developed an antibody for 6-sulfo sialyl Lewis x glycan.
\nhttps:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10514285\/<\/a><\/p>\n

To obtain an anti-6-sulfo sLex mAb, GlcNAc6ST-1\/-2 DKO mice were immunized with Chinese hamster ovary (CHO) cells stably expressing 6-sulfo sLex that had been transiently transfected with an expression vector encoding Cmah. <\/p>\n

The purified antibody named SF1 was subjected to glycan array analysis using CFG Glyucan microarray, to determine the glycan-binding specificity of SF1 in detail. It was confirmed that SF1 specifically bound to 6-sulfo sLex (Glycan #253) among all glycans on the array as follows.<\/p>\n

\"\"<\/a><\/p>\n

Lymphocyte homing to peripheral lymph nodes is critically dependent on the interaction between the homing receptor, L-selectin, expressed on the surface of lymphocytes and 6-sulfo sialyl Lewis x expressed on the surface of specialized endothelial venules. Since SF1 significantly blocks lymphocyte homing, future studies using SF1 will advance our understanding of the role of 6-sulfo sLex in disease including rheumatoid arthritis, Sj\u00f6gren syndrome, and ulcerative colitis.<\/p>\n","protected":false},"excerpt":{"rendered":"

A group from Laboratory of Microbiology and Immunology, Graduate School of Pharmaceutical Sciences, Chiba Univ<\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-13616","post","type-post","status-publish","format-standard","hentry","category-technology-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/13616","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=13616"}],"version-history":[{"count":1,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/13616\/revisions"}],"predecessor-version":[{"id":13617,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/13616\/revisions\/13617"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=13616"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=13616"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=13616"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}