{"id":12162,"date":"2023-01-23T20:31:27","date_gmt":"2023-01-23T11:31:27","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=12162"},"modified":"2023-01-23T20:32:51","modified_gmt":"2023-01-23T11:32:51","slug":"characterization-of-n-glycoproteins-from-human-tear-films","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/characterization-of-n-glycoproteins-from-human-tear-films\/","title":{"rendered":"Characterization of N-glycoproteins from human tear films"},"content":{"rendered":"<p>A group from Department of Experimental and Translational Ophthalmology, University Medical Center, Johannes Gutenberg University, Mainz, Germany has reported about characterization of N-glycoproteins from human tear films.<br \/>\n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36677706\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/36677706\/<\/a><\/p>\n<p>The main objective of this study was to develop a lectin-based affinity purification method for the enrichment of glycoproteins\/glycopeptides from human tear film and the MS-based detection as well as localization of their specific N-glycosylation sites. <\/p>\n<p>The best-performing multi-lectin column system (comprising the four lectins ConA, JAC, WGA, and UEA I, termed 4L) was applied to glycopeptide enrichment from human tear sample digests. As the main result, a total of 26 N glycosylation sites of 11 N-glycoproteins (LIF, PIGR, IGHA2, IGHA1, AZGP1, LACRT, JCHAIN, PIP, TF, HP, and SERPINA1) was identified in the tear sample pools of healthy individuals.<\/p>\n<p>This result will serve as an important N-glycoprotein reference map for future studies focusing on the ocular surface and will help to unravel the complex regulatory functions of N-glycoproteins in maintaining tear film stability and the ocular defense system.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A group from Department of Experimental and Translational Ophthalmology, University Medical Center, Johannes G<\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/characterization-of-n-glycoproteins-from-human-tear-films\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70],"tags":[],"class_list":["post-12162","post","type-post","status-publish","format-standard","hentry","category-nature-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/12162","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=12162"}],"version-history":[{"count":1,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/12162\/revisions"}],"predecessor-version":[{"id":12163,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/12162\/revisions\/12163"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=12162"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=12162"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=12162"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}