{"id":10710,"date":"2022-10-26T12:31:44","date_gmt":"2022-10-26T03:31:44","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=10710"},"modified":"2022-10-27T06:50:39","modified_gmt":"2022-10-26T21:50:39","slug":"interactions-between-galectins-and-o-mannosylated-core-m1-glycopeptides-of-%ce%b1-dystroglycan","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/interactions-between-galectins-and-o-mannosylated-core-m1-glycopeptides-of-%ce%b1-dystroglycan\/","title":{"rendered":"Interactions between galectins and O-mannosylated core M1 glycopeptides of \u03b1-dystroglycan"},"content":{"rendered":"<p>A group from Frontier Research Center for Advanced Material and Life Science, Hokkaido University, Sapporo, Japan, etc. has reported about interactions between galectins and O-mannosylated glycopeptides of \u03b1-dystroglycan, especially focusing on its core M1 structure.<br \/>\n<a href=\"https:\/\/www.nature.com\/articles\/s41598-022-22758-0\">https:\/\/www.nature.com\/articles\/s41598-022-22758-0<\/a><\/p>\n<p>The O-linked mannose (O-Man) exists in a limited number of proteins that are required for normal development and have vital functions in muscle and neural physiology. The \u03b1-dystroglycan (\u03b1-DG) is the extracellular component of dystroglycan (DG), and is the most extensively studied mammalian O-Man glycoprotein. It is ubiquitously expressed in the skeletal muscles and the brain and is associated with cell adhesion, muscle integrity, and neurological development. \u03b1-DG possesses unique glycans, LacNac-terminated three kind of core structures (M1, M2, and M3), in its mucin (MUC)\u2013like domain.<\/p>\n<p><a href=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/10\/O-mannosylated-glycans.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-10704\" src=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/10\/O-mannosylated-glycans-300x157.png\" alt=\"\" width=\"300\" height=\"157\" srcset=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/10\/O-mannosylated-glycans-300x157.png 300w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/10\/O-mannosylated-glycans-230x121.png 230w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/10\/O-mannosylated-glycans-350x183.png 350w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/10\/O-mannosylated-glycans.png 433w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p>In this study, it was shown that Human Gal-1, -4, and -9 (except -3) can strongly recognize O-Man LacNAc-terminated glycoconjugates, and the presence of an \u03b12,3-sialylated terminus led to a major reduction in the affinity of galectin, suggesting that this type of extension can fine-tune galectin activity towards this type of O-Man glycans. These interactions were significantly inhibited by lactose, establishing that the \u03b1-DG core M1-type glycans bind to the canonical sugar-binding site (S-face) of galectin, thus serving as a receptor for galectins.<\/p>\n<p>And further, it was shown in microarray experiments that Gal-1 revealed trans-bridging capabilities, linking laminin-111, -121, -211, and -221 (but little -511) and core M1 \u03b1-DG glycopeptides as shown below, providing a new insight on the therapeutic application of this galectin in muscular dystrophy.<\/p>\n<p><a href=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/10\/O-Man-microarray-with-Laminin-plus-galectin.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-10707\" src=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/10\/O-Man-microarray-with-Laminin-plus-galectin-118x300.png\" alt=\"\" width=\"118\" height=\"300\" srcset=\"https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/10\/O-Man-microarray-with-Laminin-plus-galectin-118x300.png 118w, https:\/\/www.emukk.com\/WP\/wp-content\/uploads\/2022\/10\/O-Man-microarray-with-Laminin-plus-galectin.png 189w\" sizes=\"auto, (max-width: 118px) 100vw, 118px\" \/><\/a><br \/>\nFluorescence images of M1 glycoconjugates microarrays with laminins plus galectins<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A group from Frontier Research Center for Advanced Material and Life Science, Hokkaido University, Sapporo, Ja<\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/interactions-between-galectins-and-o-mannosylated-core-m1-glycopeptides-of-%ce%b1-dystroglycan\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70,7],"tags":[],"class_list":["post-10710","post","type-post","status-publish","format-standard","hentry","category-nature-en","category-technology-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/10710","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=10710"}],"version-history":[{"count":5,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/10710\/revisions"}],"predecessor-version":[{"id":10721,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/10710\/revisions\/10721"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=10710"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=10710"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=10710"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}