{"id":10527,"date":"2022-10-17T11:02:28","date_gmt":"2022-10-17T02:02:28","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?p=10527"},"modified":"2022-10-17T11:05:10","modified_gmt":"2022-10-17T02:05:10","slug":"modification-of-glycan-binding-specificity-of-e-selectin-from-slex-to-6%e2%80%b2-sulfo-slex-with-double-mutations-e92a-e107a","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/modification-of-glycan-binding-specificity-of-e-selectin-from-slex-to-6%e2%80%b2-sulfo-slex-with-double-mutations-e92a-e107a\/","title":{"rendered":"Modification of Glycan binding Specificity of E-selectin from sLex to 6\u2032-sulfo-sLex with double mutations E92A\/E107A"},"content":{"rendered":"

A group from Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, etc. has reported that the specificity of E-selectin could be modified from sLex to 6\u2032-sulfo-sialyl Lewis X with introducing double mutations.
\nhttps:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9564326\/<\/a><\/p>\n

Although lectins are often used to detect glycans, their application to sulfated glycans is challenging due to the paucity of sulfate-recognizing lectins as well as their broad or mixed specificities.<\/p>\n

In this work, the binding specificity of E-selectin was modified by removing destabilizing steric and electrostatic interactions between the 6\u2032-sulfate and E92 and E107 with E92A\/E107A mutations, to show binding specificity to 6\u2032-sulfo-sialyl Lewis X (6\u2032-sulfo-sLex). As is known, E-selectin shows specific binding to non-sulfated ligand, sLex.
\nThis new specificity mimics that of the unrelated protein Siglec-8, for which 6\u2032-sulfo-sLex is its preferred ligand.<\/p>\n

\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

A group from Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, etc. has reported <\/p><\/div>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-10527","post","type-post","status-publish","format-standard","hentry","category-technology-en"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t