{"id":1044,"date":"2020-10-16T14:10:08","date_gmt":"2020-10-16T05:10:08","guid":{"rendered":"http:\/\/www.emukk.com\/WP\/?p=1040"},"modified":"2020-10-16T17:06:07","modified_gmt":"2020-10-16T08:06:07","slug":"using-crispr-cas12a-to-detect-new-coronavirus-covid-19sars-cov-2-adrismation-effect-using-mn2","status":"publish","type":"post","link":"https:\/\/www.emukk.com\/WP\/en\/using-crispr-cas12a-to-detect-new-coronavirus-covid-19sars-cov-2-adrismation-effect-using-mn2\/","title":{"rendered":"Using CRISPR\/Cas12a to detect new coronavirus (COVID-19:SARS-CoV-2): Sensitization effect adding Mn2+"},"content":{"rendered":"<p>CRISPR\/Cas9 won the 2020 Nobel Prize in Chemistry about a week ago.<br \/>\nWe have found several papers that used this technique as a high sensitive detection method for the new coronavirus. The following paper reports that detection using CRISPR\/Cas12a could be further 13 times more sensitive by adding Mn2+.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7536916\/#advs2020-bib-0005\">https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7536916\/#advs2020-bib-0005<\/a><\/p>\n<p>A brief description of the protocol is as follows:<\/p>\n<ul>\n<li>Extract an RNA sample from a sample.<\/li>\n<li>Make cDNA using reverse transcription, and amplify by PCR (dsDNA is made).<\/li>\n<li>Pre-design crisper RNA (crRNA) using RNA sequences specific to the E protein of the new coronavirus.<\/li>\n<li>Add Cas12a enzyme and crRNA to the dsDNA sample, the new coronavirus-specific sequence is cut out when it is in the sample dsDNA.<\/li>\n<li>Thus, since the inhibitory interaction between the fluorescent probe and the quencher that have been incorporated in the PCR process is lost, the fluorescence comes out by applying the excitation light.<\/li>\n<\/ul>\n<p>Of course, this fluorescence is read by a detector, but it can be confirmed by the eyes.<br \/>\nThe addition of Mn2+ to this system increased the sensitivity by 13 times.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>CRISPR\/Cas9 won the 2020 Nobel Prize in Chemistry about a week ago. We have found several papers that used thi<\/p><\/div>\n<div class=\"blog-btn\"><a href=\"https:\/\/www.emukk.com\/WP\/en\/using-crispr-cas12a-to-detect-new-coronavirus-covid-19sars-cov-2-adrismation-effect-using-mn2\/\" class=\"home-blog-btn\">\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-1044","post","type-post","status-publish","format-standard","hentry","category-technology-en"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/1044","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=1044"}],"version-history":[{"count":6,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/1044\/revisions"}],"predecessor-version":[{"id":1051,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/posts\/1044\/revisions\/1051"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=1044"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/categories?post=1044"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/tags?post=1044"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}