{"id":5399,"date":"2022-10-09T17:50:34","date_gmt":"2022-10-09T08:50:34","guid":{"rendered":"https:\/\/www.emukk.com\/WP\/?page_id=5399"},"modified":"2024-07-02T17:19:20","modified_gmt":"2024-07-02T08:19:20","slug":"%e3%83%ac%e3%82%af%e3%83%81%e3%83%b3%e3%83%9e%e3%82%a4%e3%82%af%e3%83%ad%e3%82%a2%e3%83%ac%e3%82%a4%e3%81%ae%e6%a8%99%e6%ba%96%e3%83%97%e3%83%ad%e3%83%88%e3%82%b3%e3%83%ab","status":"publish","type":"page","link":"https:\/\/www.emukk.com\/WP\/products-services-2\/%e3%83%ac%e3%82%af%e3%83%81%e3%83%b3%e3%83%9e%e3%82%a4%e3%82%af%e3%83%ad%e3%82%a2%e3%83%ac%e3%82%a4%e3%81%ae%e6%a8%99%e6%ba%96%e3%83%97%e3%83%ad%e3%83%88%e3%82%b3%e3%83%ab\/","title":{"rendered":"\u30ec\u30af\u30c1\u30f3\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u6a19\u6e96\u30d7\u30ed\u30c8\u30b3\u30eb"},"content":{"rendered":"\n

\u6e96\u5099\u3059\u308b\u8a66\u85ac\u985e<\/h3>\n\n\n\n
    \n
  1. PBS: 0.01M Phosphate-Buffered Saline\u3001pH 7.2\u20137.4<\/li>\n\n\n\n
  2. PBS-Tx: PBS containing 1% (v\/v) Triton X-100<\/li>\n\n\n\n
  3. Tris-buffered saline\uff08TBS\uff09: 25 mM Tris\u3001140 mM NaCl\u30013 mM KCl, pH 7.5<\/li>\n\n\n\n
  4. Micro BCA Protein Assay Reagent Kit\uff08Thermo Fisher Scientific Inc., Cat. #23235\uff09<\/a><\/li>\n\n\n\n
  5. Cy3 Mono-Reactive Dye Pack 1 mg \u00d7 5\uff08GE Healthcare – Life Sciences, Cat. #PA23001\uff09<\/a><\/li>\n\n\n\n
  6. Dimethyl formamide\uff08DMF\uff09: dehydrated<\/li>\n\n\n\n
  7. Zeba Desalt Spin Columns\u30010.5 mL \uff08Thermo Fisher Scientific Inc., Cat. #89882\uff09<\/a><\/li>\n\n\n\n
  8. Probing Solution\uff08\u30a8\u30e0\u30c3\u30af\u3078\u554f\u3044\u5408\u308f\u305b\u3001\u53c2\u7167\u8ad6\u6587<\/a>\u306b\u7d44\u6210\u306e\u8a18\u8f09\u3042\u308a\uff09<\/li>\n\n\n\n
  9. \u30ad\u30e0\u30c6\u30c3\u30af\u30b9\u30fb\u30db\u30ef\u30a4\u30c8\uff08CRECIA\u3001Cat.#63200\uff09<\/a><\/li>\n\n\n\n
  10. IgG from human serum (h-IgG) \uff08Sigma-Aldrich, Cat. #I8640\uff09<\/a><\/li>\n\n\n\n
  11. Cy3-Streptavidin\u30011 mg\/mL\uff08GE Healthcare – Life Sciences, Cat. #PA43001\uff09<\/a><\/li>\n\n\n\n
  12. Mem-PER Plus Membrane Protein Extraction Kit\uff08Thermo Fisher Scientific Inc., Cat. #89842\uff09<\/a><\/li>\n\n\n\n
  13. \u30e2\u30ec\u30ad\u30e5\u30e9\u30fc\u30b7\u30fc\u30d6\uff08Sigma-Aldrich, Cat.#208604\uff09<\/a><\/li>\n<\/ol>\n\n\n\n

    \u4f7f\u7528\u3059\u308b\u88c5\u7f6e<\/h3>\n\n\n
    \n
    \"\"\n\t\t\t\n\t\t\t\t\n\t\t\t<\/svg>\n\t\t<\/button>
    \u30d1\u30c3\u30ad\u30f3\u30b0\u3055\u308c\u305f\u30ec\u30af\u30c1\u30f3\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4<\/figcaption><\/figure>\n<\/div>\n\n
    \n
    \"\"\n\t\t\t\n\t\t\t\t\n\t\t\t<\/svg>\n\t\t<\/button>
    GlycoStation Reader 2300 \uff08GSR2300\uff09<\/figcaption><\/figure>\n<\/div>\n\n\n
      \n
    1. \u51b7\u5374\u5c0f\u578b\u9060\u5fc3\u6a5f\uff08MX-301: Tomy Seiko Co., Ltd.\uff09<\/li>\n\n\n\n
    2. \u30d7\u30ec\u30fc\u30c8\u30ea\u30fc\u30c0\u30fc\uff08SpectraMax M5: Molecular Devices\uff09<\/li>\n\n\n\n
    3. \u30ec\u30af\u30c1\u30f3\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4 Ver.1.0 and Ver.2.0\uff08Precision System Science Co., Ltd.\u3001\u3054\u4e0d\u660e\u70b9\u304c\u3042\u308c\u3070\u30a8\u30e0\u30c3\u30af\u3078\uff09<\/li>\n\n\n\n
    4. GlycoStation Reader 2300, GlycoSuperLite 2200 \uff08\u30a8\u30e0\u30c3\u30af\uff09<\/li>\n\n\n\n
    5. GlycoStation ToolsPro Ver.3.0 \uff08\u30a8\u30e0\u30c3\u30af\uff09<\/li>\n\n\n\n
    6. \u771f\u7a7a\u9060\u5fc3\u4e7e\u71e5\u6a5f\uff08UT-1000, Cat #CVE-200D: Tokyo Rikakikai Co. Ltd. [EYELA]\uff09<\/li>\n\n\n\n
    7. \u30a4\u30f3\u30d3\u30c8\u30ed\u30b7\u30a7\u30fc\u30ab\u30fc\uff08Wave-PR, TAITEC Corp.\uff09<\/li>\n<\/ol>\n\n\n\n

      \u30bf\u30f3\u30d1\u30af\u8cea\u306e\u62bd\u51fa<\/h3>\n\n\n\n
        \n
      1. Mem-PER Plus Membrane Protein Extraction Kit\u3092\u4f7f\u7528\u3059\u308b<\/li>\n\n\n\n
      2. \u305d\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u3064\u3044\u3066\u306f\u3001Thermofisher\u306e\u8a72\u5f53\u30da\u30fc\u30b8\u3092\u53c2\u7167\u306e\u3053\u3068<\/a><\/li>\n<\/ol>\n\n\n\n

        \u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5b9a\u91cf<\/h3>\n\n\n\n
          \n
        1. Micro BCA Protein Assay Reagent Kit\u3092\u4f7f\u7528\u3059\u308b<\/li>\n\n\n\n
        2. \u30c1\u30e5\u30fc\u30d6\u30e9\u30c3\u30af\u306b1.5-mL\u306e\u30de\u30a4\u30af\u30ed\u30c1\u30e5\u30fc\u30d6\u3092\u7f6e\u304d\u3001BSA\u3068\u30e9\u30d9\u30ea\u30f3\u30b0\u3057\u3001240 \u00b5L \u306ePBS\u3092\u30a2\u30d7\u30e9\u30a4\u3059\u308b<\/li>\n\n\n\n
        3. 10 \u00b5L \u306eBSA \u6eb6\u6db2\uff082 mg\/mL\uff09\u3092\u5148\u306eBSA\u30c1\u30e5\u30fc\u30d6\u306b\u30a2\u30d7\u30e9\u30a4\u3057\u3001\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3057\u306680 \u00b5g\/mL\u306eBSA\u6eb6\u6db2\u3092\u4f5c\u308b<\/li>\n\n\n\n
        4. BSA\uff0880 \u00b5g\/mL\uff09\u30c1\u30e5\u30fc\u30d6\u3068\u3001\u6e2c\u5b9a\u3057\u305f\u3044\u30b5\u30f3\u30d7\u30eb\u304c\u5165\u3063\u305f\u30c1\u30e5\u30fc\u30d6\u3092\u6c37\u4e0a\u306b\u304a\u304f<\/li>\n\n\n\n
        5. 96-\u7a74MTP\u306b\u5e0c\u91c8\u500d\u7387\uff1d1\/2\u306b\u3066\u3001BSA\u306e\u5e0c\u91c8\u7cfb\u5217\u3092\u4f5c\u6210\u3059\u308b\u300280 \u00b5g\/mL \u306e\u6fc3\u5ea6\u304b\u3089\u3001\u305d\u306e1\/128\u500d\u306e\u6fc3\u5ea6\u307e\u3067\uff08\u6eb6\u6db2\u91cf= 100 \u00b5L\uff09<\/li>\n\n\n\n
        6. \u6e2c\u5b9a\u3057\u305f\u3044\u30b5\u30f3\u30d7\u30eb\u306b\u3064\u3044\u3066\u3082\u3001\u540c\u69d8\u306a\u5e0c\u91c8\u7cfb\u5217\u3092\u540c\u305896-\u7a74MTP\u306b\u4f5c\u6210\u3059\u308b<\/li>\n\n\n\n
        7. 2.5 mL \u306e Micro BCA Reagent A\uff08MA\uff09\u30012.4 mL \u306e Micro BCA Reagent B\uff08MB\uff09\u305d\u3057\u3066 100 \u00b5L \u306e Micro BCA Reagent C\uff08MC\uff09\u309215-mL \u306e\u30c1\u30e5\u30fc\u30d6\u306b\u30a2\u30d7\u30e9\u30a4\u3057\u3001\u826f\u304f\u6df7\u5408\u3059\u308b\uff08\u3053\u308c\u3092WR\u3068\u547c\u3076\u3053\u3068\u306b\u3059\u308b\uff09<\/li>\n\n\n\n
        8. 100 \u00b5L \u306e WR\u3092\u3059\u3079\u3066\u306eMTP\u306e\u30a6\u30a7\u30eb\u306b\u30a2\u30d7\u30e9\u30a4\u3059\u308b<\/li>\n\n\n\n
        9. \u30d7\u30ec\u30fc\u30c8\u30b7\u30fc\u30e9\u30fc\u3067MTP\u3092\u30b7\u30fc\u30eb\u3059\u308b<\/li>\n\n\n\n
        10. 37\u00b0C\u3067\uff12\u6642\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3059\u308b<\/li>\n\n\n\n
        11. \u30d7\u30ec\u30fc\u30c8\u30b7\u30fc\u30e9\u30fc\u3092\u5265\u304c\u3059<\/li>\n\n\n\n
        12. 562 nm \u306e\u5438\u5149\u5ea6\u3092\u30d7\u30ec\u30fc\u30c8\u30ea\u30fc\u30c0\u3067\u6e2c\u5b9a\u3057\u3001BSA\u304b\u3089\u5f97\u3089\u308c\u305f\u691c\u91cf\u7dda\u304b\u3089\u6e2c\u5b9a\u3057\u305f\u3044\u30b5\u30f3\u30d7\u30eb\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u6fc3\u5ea6\u3092\u8a08\u7b97\u3059\u308b<\/li>\n<\/ol>\n\n\n\n

          Cy3\u306e\u30e9\u30d9\u30ea\u30f3\u30b0<\/h3>\n\n\n\n
            \n
          1. \u30bf\u30f3\u30d1\u30af\u8cea\u6fc3\u5ea6\u306e\u6e2c\u5b9a\u7d50\u679c\u304b\u3089\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u6fc3\u5ea6\u3092PBS\u3092\u30a2\u30d7\u30e9\u30a4\u3057\u306650 \u03bcg\/mL \u306b\u8abf\u6574\u3059\u308b\uff08\u6ce8\u610f\u4e8b\u9805 1\uff09<\/li>\n\n\n\n
          2. 100 \u03bcg \u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30e9\u30d9\u30ea\u30f3\u30b0\u3059\u308b\u306b\u5fc5\u8981\u306a\u91cf\u306b\u5c0f\u5206\u3051\u3057\u305fCy3\u304c\u5165\u3063\u3066\u3044\u308b\u30c1\u30e5\u30fc\u30d6\u306b\u300120 \u00b5L \u306e\u30b5\u30f3\u30d7\u30eb\u3092\u30a2\u30d7\u30e9\u30a4\u3059\u308b\uff08\u6ce8\u610f\u4e8b\u9805 2\uff09<\/li>\n\n\n\n
          3. \u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u826f\u304f\u6df7\u5408\u3059\u308b<\/li>\n\n\n\n
          4. \u30c1\u30e5\u30fc\u30d6\u306e\u5074\u9762\u306b\u3064\u3044\u3066\u3044\u308b\u6db2\u4f53\u3092\u9060\u5fc3\u3057\u3066\u5168\u3066\u843d\u3068\u3059<\/li>\n\n\n\n
          5. \u30c1\u30e5\u30fc\u30d6\u3092\u30a2\u30eb\u30df\u7b94\u306a\u3069\u3067\u906e\u5149\u3057\u300125\u00b0C\u306b\u3066\uff11\u6642\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3059\u308b<\/li>\n<\/ol>\n\n\n\n

            \u30d5\u30ea\u30fc Cy3\u3092\u9664\u53bb\u3059\u308b\u305f\u3081\u306e\u30b2\u30eb\u308d\u904e\u30ab\u30e9\u30e0<\/h3>\n\n\n
            \n
            \"\"\n\t\t\t\n\t\t\t\t\n\t\t\t<\/svg>\n\t\t<\/button>
            \u4f7f\u7528\u5f8c\u306e\u30b2\u30eb\u308d\u904e\u30ab\u30e9\u30e0\u306e\u72b6\u614b<\/figcaption><\/figure>\n<\/div>\n\n\n
              \n
            1. Zeba desalt spin columns\uff080.5 mL\uff09\u3092\u7528\u610f\u3059\u308b<\/li>\n\n\n\n
            2. \u30ab\u30e9\u30e0\u306e\u305d\u3053\u306b\u3042\u308b\u30d7\u30e9\u30b0\u3092\u6369\u3058\u308a\u53d6\u308a\u3001\u30ad\u30e3\u30c3\u30d7\u3092\u7de9\u3081\u308b\uff08\u6ce8\u610f\u4e8b\u9805 3\uff09<\/li>\n\n\n\n
            3. \u30ab\u30e9\u30e0\u30922.0 mL\u306e\u30de\u30a4\u30af\u30ed\u30c1\u30e5\u30fc\u30d6\u306b\u306f\u3081\u308b<\/li>\n\n\n\n
            4. 1,500 \u00d7g \u306b\u30661\u5206\u9593\u9060\u5fc3\u3057\u3001\u30ab\u30e9\u30e0\u5185\u306e\u6eb6\u6db2\u3092\u9664\u53bb\u3059\u308b<\/li>\n\n\n\n
            5. \u9060\u5fc3\u3067\u30ec\u30b8\u30f3\u306f\u659c\u3081\u306b\u306a\u308b\u304c\u3001\u305d\u306e\u659c\u3081\u306b\u306a\u3063\u305f\u30ec\u30b8\u30f3\u306e\u9802\u4e0a\u90e8\u5074\u306e\u4f4d\u7f6e\u3092\u30ab\u30e9\u30e0\u306b\u30de\u30fc\u30af\u3059\u308b<\/li>\n\n\n\n
            6. \u9060\u5fc3\u6a5f\u306b\u30ab\u30e9\u30e0\u3092\u30bb\u30c3\u30c8\u3059\u308b\u6642\u306f\u3001\u305d\u306e\u30de\u30fc\u30af\u304c\u5e38\u306b\u5916\u5074\u306b\u306a\u308b\u3088\u3046\u306b\u3057\u3066\u30bb\u30c3\u30c8\u3059\u308b<\/li>\n\n\n\n
            7. 300 \u03bcL \u306e TBS \u3092\u30ab\u30e9\u30e0\u306b\u30a2\u30d7\u30e9\u30a4\u3057\u30011,500 \u00d7g \u3067\uff11\u5206\u9593\u9060\u5fc3\u3057\u3001TBS\u3092\u9664\u53bb\u3059\u308b<\/li>\n\n\n\n
            8. \u3053\u306e\u30b9\u30c6\u30c3\u30d7\u3092\u5408\u8a083\u56de\u7e70\u308a\u8fd4\u3059<\/li>\n\n\n\n
            9. \u65b0\u3057\u3044\u30b5\u30f3\u30d7\u30eb\u56de\u53ce\u7528\u306e\u30c1\u30e5\u30fc\u30d6\u306b\u30ab\u30e9\u30e0\u3092\u306f\u3081\u3001\u30ad\u30e3\u30c3\u30d7\u3092\u9664\u53bb\u3057\u3066\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u5168\u91cf\uff0820 \u03bcL\uff09\u3092\u30ec\u30b8\u30f3\u306e\u4e0a\u90e8\u5074\u304b\u3089\u614e\u91cd\u306b\u6ce8\u304e\u8fbc\u3080<\/li>\n\n\n\n
            10. \u30b5\u30f3\u30d7\u30eb\u304c\u5b8c\u5168\u306b\u30ec\u30b8\u30f3\u306b\u5438\u53ce\u3055\u308c\u305f\u5f8c\u300125 \u03bcL \u306e TBS\u3092\u30b9\u30bf\u30c3\u30ab\u30fc\u3068\u3057\u3066\u30ec\u30b8\u30f3\u306b\u30a2\u30d7\u30e9\u30a4\u3059\u308b<\/li>\n\n\n\n
            11. 1,500 \u00d7g \u30672\u5206\u9593\u9060\u5fc3\u3057\u3001Cy3\u304c\u30e9\u30d9\u30ea\u30f3\u30b0\u3055\u308c\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u56de\u53ce\u3059\u308b<\/li>\n\n\n\n
            12. \u4f7f\u7528\u3057\u305f\u30ab\u30e9\u30e0\u306f\u5ec3\u68c4\u3059\u308b<\/li>\n<\/ol>\n\n\n\n

              \u30ec\u30af\u30c1\u30f3\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306b\u30a2\u30d7\u30e9\u30a4<\/h3>\n\n\n
              \n
              \"\"\n\t\t\t\n\t\t\t\t\n\t\t\t<\/svg>\n\t\t<\/button>
              \u86cd\u5149\u6a19\u8b58\u3055\u308c\u305f\u30b5\u30f3\u30d7\u30eb\u3001\u8584\u3044\u30d4\u30f3\u30af\u8272\u306b\u898b\u3048\u308b\u306e\u304c\u6b63\u3057\u304f\u30e9\u30d9\u30ea\u30f3\u30b0\u3055\u308c\u305f\u30b5\u30f3\u30d7\u30eb\u306e\u7279\u5fb4\u3067\u3059<\/figcaption><\/figure>\n<\/div>\n\n\n
                \n
              1. Cy3\u304c\u30e9\u30d9\u30ea\u30f3\u30b0\u3055\u308c\u305f\u30b5\u30f3\u30d7\u30eb\u91cf\u3092\u30d4\u30da\u30c3\u30c8\u3067\u78ba\u8a8d\u3059\u308b\uff0845\u03bcL\u306e\u306f\u305a\uff09<\/li>\n\n\n\n
              2. 455 \u03bcL \u306e Probing Solution\u3092\u30a2\u30d7\u30e9\u30a4\u3057\u3001500 \u03bcL\u3078\u30e1\u30b9\u30a2\u30c3\u30d7\u3059\u308b\u3002\u3053\u308c\u306b\u3088\u3063\u3066\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u6fc3\u5ea6\u306f\u30012 \u03bcg\/mL\u3068\u306a\u308b<\/li>\n\n\n\n
              3. 2,000\u30011,000\u3001500\u3001250\u3001125\u300162.5\u300131.25 ng\/mL\u3068\u3044\u30467\u6bb5\u968e\u306e\u5e0c\u91c8\u7cfb\u5217\u3092Probing Solution\u3092\u7528\u3044\u3066\u4f5c\u6210\u3059\u308b\uff08\u305d\u308c\u305e\u308c\u3001100\u03bcL\u91cf\u3068\u3059\u308b\uff09.<\/li>\n\n\n\n
              4. \u30ec\u30af\u30c1\u30f3\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3092\u51b7\u51cd\u5eab\uff08\uff0d20\u2103\uff09\u304b\u3089\u53d6\u308a\u51fa\u3057\u3001\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3BOX\u306e\u4e2d\u306b\u7f6e\u304f<\/li>\n\n\n\n
              5. 8\u9023\u306e\u30de\u30eb\u30c1\u30d4\u30da\u30c3\u30c8\u3092\u4f7f\u3063\u3066\u3001100 \u03bcL \u306e Probing Solution \u3092\u30ec\u30af\u30c1\u30f3\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u30a6\u30a7\u30eb\u306b\u540c\u6642\u306b\u30a2\u30d7\u30e9\u30a4\u3059\u308b<\/li>\n\n\n\n
              6. \u6570\u79d2\u9593\u30c1\u30c3\u30d7\u3092\u8efd\u304f\u63fa\u3059\u3063\u3066\u304b\u3089\u3001Probing Solution\u3092\u6368\u3066\u3001\u30ad\u30e0\u30c6\u30c3\u30af\u30b9\u306e\u4e0a\u306b\u30c1\u30c3\u30d7\u3092\u88cf\u8fd4\u3057\u3066\u7f6e\u304d\u3001\u4f59\u5206\u306aProbing Solution\u3092\u5438\u308f\u305b\u308b<\/li>\n\n\n\n
              7. \u30c1\u30c3\u30d7\u3092\u8868\u306b\u3057\u3066\u3001\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3BOX\u306b\u7f6e\u304d\u3001\u30c1\u30c3\u30d7\u8868\u9762\u304c\u4e7e\u304b\u306a\u3044\u5185\u306b\u3001\u518d\u3073100 \u03bcL \u306e Probing Solution\u3092\u3059\u3079\u3066\u306e\u30a6\u30a7\u30eb\u306b\u30a2\u30d7\u30e9\u30a4\u3059\u308b<\/li>\n\n\n\n
              8. \u3053\u308c\u3092\u5408\u8a083\u56de\u7e70\u308a\u8fd4\u3059<\/li>\n\n\n\n
              9. Probing Solution\u3092\u6368\u3066\u3001\u4f5c\u6210\u3057\u305f7\u6bb5\u968e\u306e\u5e0c\u91c8\u7cfb\u5217\uff08100 \u03bcL\uff09\u3092\u30ec\u30af\u30c1\u30f3\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\uff17\u3064\u3042\u308b\u30a6\u30a7\u30eb\u306b\u7d20\u65e9\u304f\u30a2\u30d7\u30e9\u30a4\u3059\u308b<\/li>\n\n\n\n
              10. \u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3BOX\u306e\u5e95\u306b\u7d14\u6c34\u3092\u5c11\u3005\u5165\u308c\u3001\u30ec\u30af\u30c1\u30f3\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3092\u4e26\u3079\u3066\u304b\u3089\u84cb\u3092\u3057\u3066\u5b8c\u5168\u306b\u30b7\u30fc\u30eb\u3057\u3001\u30a2\u30eb\u30df\u7b94\u3092\u304b\u3076\u305b\u3066\u906e\u5149\u3057\u3066\u30a4\u30f3\u30d3\u30c8\u30ed\u30b7\u30a7\u30fc\u30ab\u30fc\u306e\u4e0a\u306b\u7f6e\u304d\u300120\u00b0C \u306716\u6642\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u3092\u884c\u3046<\/li>\n<\/ol>\n\n\n\n

                \u30c7\u30fc\u30bf\u306e\u53d6\u5f97\u3068\u89e3\u6790\u6cd5<\/h3>\n\n\n\n

                GlycoStation Reader 2300\u3084GlycoSuperLite 2200\u3092\u7528\u3044\u3066\u30ec\u30af\u30c1\u30f3\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3092\u30b5\u30f3\u30d7\u30eb\u6eb6\u6db2\u3092\u6368\u3066\u305a\u306b\u305d\u306e\u307e\u307e\u306e\u72b6\u614b\u3067\u30b9\u30ad\u30e3\u30f3\u3059\u308b\uff08\u6ce8\u610f\u4e8b\u9805 4\uff09\u3002\u30a2\u30ec\u30a4\u3092\u30b9\u30ad\u30e3\u30ca\u30fc\u306e\u30b9\u30c6\u30fc\u30b8\u306b\u30bb\u30c3\u30c8\u3057\u3066\u30b9\u30ad\u30e3\u30f3\u3059\u308b\u524d\u306b\u3001\u30b9\u30e9\u30a4\u30c9\u30b0\u30e9\u30b9\u306e\u88cf\u9762\u309270%\u30a8\u30bf\u30ce\u30fc\u30eb\u3067\u62ed\u304d\u3001\u6c5a\u308c\u3092\u9664\u53bb\u3059\u308b\u3053\u3068\u3092\u5fd8\u308c\u306a\u3044\u3088\u3046\u306b\u3059\u308b\u3002\u30b9\u30ad\u30e3\u30f3\u3057\u305f\u86cd\u5149\u753b\u50cf\uff08TIFF\u753b\u50cf\uff09\u3092GlycoStation ToolsPro Ver.3.0\u3092\u7528\u3044\u3066\u6570\u5024\u5316\u3057\u3001\u89e3\u6790\u3092\u884c\u3046\u3002<\/p>\n\n\n\n

                \"\"
                \u30a2\u30d9\u30ec\u30fc\u30b8\u898f\u683c\u5316\u5f8c\u306e\u6bd4\u8f03\u7cd6\u9396\u30d7\u30ed\u30d5\u30a1\u30a4\u30ea\u30f3\u30b0\u56f3\uff084\u30b5\u30f3\u30d7\u30eb\uff09\u306e\u4f8b<\/figcaption><\/figure>\n\n\n\n
                  \n
                1. TIFF\u753b\u50cf\u3092\u8aad\u307f\u8fbc\u3093\u3067\u30b0\u30ea\u30c3\u30c9\u3092\u767a\u751f\u3055\u305b\u3001\u30b9\u30dd\u30c3\u30c8\u306e\u5f37\u5ea6\u3092\u6570\u5024\u5316\u3057\u3066\u3001Net Intensity\u3092\u5f97\u308b\uff0816 bits\uff09<\/li>\n\n\n\n
                2. 2\u7a2e\u985e\u306ebackground modes\uff08Local Background mode \u3068 Global Background mode\uff09\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b<\/li>\n\n\n\n
                3. \u30b2\u30a4\u30f3\u7d71\u5408\uff08data integration\u3068\u306e\u547c\u3076\uff09\u306e\u6a5f\u80fd\u3092\u4f7f\u3063\u3066\u3001\u98fd\u548c\u3057\u3066\u3044\u308b\u30ec\u30af\u30c1\u30f3\u306e\u4fe1\u53f7\u3092\u88dc\u6b63\u3057\u3001\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u3092\u5e83\u3052\u308b\u3002\u3053\u306e\u6a5f\u80fd\u3092\u4f7f\u3046\u306b\u306f\u3001\u7570\u306a\u3063\u305f\u30b2\u30a4\u30f3\u3067\u53d6\u5f97\u3057\u305f\u6700\u4f4e\u4e8c\u679a\u306e\u86cd\u5149\u753b\u50cf\u304c\u5fc5\u8981\u3067\u3042\u308b<\/li>\n\n\n\n
                4. binding curves\uff08\u6fc3\u5ea6\u304c\u6a2a\u8ef8\u3067\u3001\u7e26\u8ef8\u304cNet Intensity\uff09\u3092\u63cf\u304d\u3001\u6bd4\u8f03\u89e3\u6790\u3092\u884c\u3046\u305f\u3081\u306e\u6700\u9069\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u6fc3\u5ea6\u3092\u9078\u3073\u51fa\u3059<\/li>\n\n\n\n
                5. \u6bd4\u8f03\u7cd6\u9396\u30d7\u30ed\u30d5\u30a1\u30a4\u30ea\u30f3\u30b0\u89e3\u6790\u306e\u56f3\u3092\u63cf\u304f\uff08\u4e00\u5ea6\u306b\u6700\u592710\u6bd4\u8f03\u3092\u884c\u3046\u3053\u3068\u304c\u53ef\u80fd\uff09<\/li>\n\n\n\n
                6. \u305d\u306e\u5834\u5408\u306b\u3001\u5e7e\u3064\u304b\u306e\u898f\u683c\u5316\u6cd5\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\uff08Raw\u3001Marker Intensity\u3001Highest Intensity\u3001Average\u3001\u7279\u5b9a\u306e\u30ec\u30af\u30c1\u30f3\u306a\u3069)<\/li>\n\n\n\n
                7. \u63cf\u3044\u305f\u56f3\u306f\u3001Copy and paste\u306b\u3066\u3001\u30af\u30ea\u30c3\u30d7\u30dc\u30fc\u30c9\u3092\u4ecb\u3057\u3066PowerPoint\u3089\u306b\u8cbc\u308a\u4ed8\u3051\u308b\u3053\u3068\u304c\u51fa\u6765\u308b<\/li>\n\n\n\n
                8. \u307e\u305f\u3001\u305d\u306e\u6570\u5024\u5316\u30c7\u30fc\u30bf\u306f\u3001Excel\u306b\u30a8\u30af\u30b9\u30dd\u30fc\u30c8\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b<\/li>\n\n\n\n
                9. \u30a8\u30af\u30b9\u30dd\u30fc\u30c8\u3057\u305f\u30c7\u30fc\u30bf\u306f\u3001SA\/DL Easy\uff08Mx\uff09\u3092\u4f7f\u3063\u3066\u3001\u7d71\u8a08\u89e3\u6790\u3084\u6df1\u5c64\u5b66\u7fd2\u3092\u304a\u3053\u306a\u3046\u3053\u3068\u304c\u53ef\u80fd\u3067\u3042\u308b<\/li>\n\n\n\n
                10. Ver3.0\u3067\u306f\u3001\u30ec\u30af\u30c1\u30f3\u540d\u306e\u7de8\u96c6\u6a5f\u80fd\u3092\u4f7f\u3063\u3066\u3069\u3093\u306a\u30ab\u30b9\u30bf\u30e0\u30a2\u30ec\u30a4\u3067\u3082\u53d6\u308a\u6271\u3046\u3053\u3068\u304c\u53ef\u80fd\u3067\u3042\u308b<\/li>\n<\/ol>\n\n\n\n

                  \u6297\u4f53\u30aa\u30fc\u30d0\u30fc\u30ec\u30a4\u6cd5<\/h3>\n\n\n\n
                    \n
                  1. Probing Solution\u3092\u7528\u3044\u3066\u30b5\u30f3\u30d7\u30eb\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u6fc3\u5ea6\u3092\u9069\u5f53\u306a\u5024\u306b\u8abf\u6574\u3059\u308b\uff08\u901a\u5e38\u30010.25 \u00b5g\/mL \u304b\u3089 2 \u00b5g\/mL\u306e\u7bc4\u56f2\uff09<\/li>\n\n\n\n
                  2. 50 \u00b5L \u306e\u30b5\u30f3\u30d7\u30eb\u3092\u30ec\u30af\u30c1\u30f3\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306b\u30a2\u30d7\u30e9\u30a4\u3059\u308b<\/li>\n\n\n\n
                  3. \u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u306b\u30a2\u30ec\u30a4\u3092\u4e26\u3079\u3001\u30a4\u30f3\u30d3\u30c8\u30ed\u30b7\u30a7\u30fc\u30ab\u30fc\u306b\u4e57\u305b\u3001\u6697\u6240\u300120\u00b0C\u306b\u3066\u30aa\u30fc\u30d0\u30fc\u30ca\u30a4\u30c8\u3067\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3059\u308b<\/li>\n\n\n\n
                  4. human IgG (h-IgG, 10 mg\/mL) \u30922 \u00b5L\/well\u3067\u30d6\u30ed\u30c3\u30ab\u30fc\u3068\u3057\u3066\u5404\u30a6\u30a7\u30eb\u306b\u30a2\u30d7\u30e9\u30a4\u3059\u308b\uff08\u6ce8\u610f\u4e8b\u9805 5\uff09<\/li>\n\n\n\n
                  5. \u30b7\u30a7\u30fc\u30ab\u30fc\u4e0a\u3067\u300120\u00b0C \u306730\u5206\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3059\u308b<\/li>\n\n\n\n
                  6. PBS-Tx \u306b\u30663\u56de\u30a6\u30a7\u30eb\u3092\u30a6\u30a9\u30c3\u30b7\u30e5\u3059\u308b\uff08100 \u00b5L\/well\uff09<\/li>\n\n\n\n
                  7. PBS-Tx \uff0848 \u00b5L\/well\uff09\u3001h-IgG (10 mg\/mL) \uff082 \u00b5L\/well\uff09\u3001\u305d\u3057\u3066\u30d3\u30aa\u30c1\u30f3\u5316\u6297\u4f53\u3092\u9069\u5207\u306a\u6fc3\u5ea6\uff084 \u00b5g\/mL \uff5e 500 ng\/mL\u306e\u7bc4\u56f2\uff09\uff0850 \u00b5L\/well\uff09\u306b\u3066\u30a2\u30d7\u30e9\u30a4\u3059\u308b<\/li>\n\n\n\n
                  8. \u30b7\u30a7\u30fc\u30ab\u30fc\u4e0a\u3067\u300120\u00b0C \u30671\u6642\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3059\u308b<\/li>\n\n\n\n
                  9. PBS-Tx \uff08100 \u00b5L\/well\uff09\u30673\u56de\u30a6\u30a7\u30eb\u3092\u30a6\u30a9\u30c3\u30b7\u30e5\u3059\u308b<\/li>\n\n\n\n
                  10. PBS-Tx \uff0846 \u00b5L\/well\uff09\u3092\u30a2\u30d7\u30e9\u30a4\u3057\u3001\u6b21\u306bCy3-Streptavidin\uff0850 \u00b5g\/mL by PBS\uff09\uff084 \u00b5L\/well\uff09\u3092\u30a2\u30d7\u30e9\u30a4\u3059\u308b<\/li>\n\n\n\n
                  11. 20\u00b0C \u306730\u5206\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3059\u308b<\/li>\n\n\n\n
                  12. PBS-Tx \uff08100 \u00b5L\/well\uff09\u30673\u56de\u30a6\u30a9\u30c3\u30b7\u30e5\u3059\u308b<\/li>\n\n\n\n
                  13. PBS-Tx \uff08 50 \u00b5L\/well\uff09\u3092\u30a2\u30d7\u30e9\u30a4\u3059\u308b<\/li>\n\n\n\n
                  14. GlycoStation Reader 2300\u3001\u6216\u3044\u306fGlycoSuperLite 2200\u3067\u30b9\u30ad\u30e3\u30f3\u3059\u308b<\/li>\n<\/ol>\n\n\n\n
                    \"\"
                    \u4ee5\u4e0b\u306f\u3001\u6ce8\u610f\u4e8b\u9805\u3067\u3059\u3002\u826f\u304f\u3054\u78ba\u8a8d\u304f\u3060\u3055\u3044\uff01<\/strong>(cartoon Style)<\/figcaption><\/figure>\n\n\n\n
                      \n
                    1. \u4ed6\u306e\u4e2d\u6027\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u985e\u3092\u7528\u3044\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u304c\u3001\u30a2\u30df\u30f3\u3092\u542b\u3093\u3060\u3082\u306e\uff08\u4f8b\u3048\u3070Tris buffer\uff09\u306f\u4f7f\u3048\u306a\u3044\u3002\u30a2\u30df\u30f3\u306fCy3-NHS ester\u3068\u53cd\u5fdc\u3057\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30e9\u30d9\u30ea\u30f3\u30b0\u52b9\u7387\u304c\u4e0b\u304c\u3063\u3066\u3057\u307e\u3046<\/li>\n\n\n\n
                    2. Cy3\uff08100 \u00b5g\/tube\uff09\u306e\u5c0f\u5206\u3051\u306e\u4ed5\u65b9\u3002\u30e2\u30ec\u30ad\u30e5\u30e9\u30fc\u30b7\u30fc\u30d6\u3092\u7528\u3044\u3066DMF\u3092\u4e00\u6669\u304b\u3051\u3066\u8131\u6c34\u3059\u308b\u3002Cy3 Mono-Reactive Dye Pack\uff081mg\uff09\u309250 \u00b5L \u306e DMF\u306b\u6eb6\u304b\u3059\u300210\u672c\u306e\u30de\u30a4\u30af\u30ed\u30c1\u30e5\u30fc\u30d6\u306bCy3-DMF\u30925 \u00b5L \u3065\u3064\u30d4\u30da\u30c3\u30c8\u3067\u53d6\u308a\u5206\u3051\u308b\u3002\u771f\u7a7a\u9060\u5fc3\u4e7e\u71e5\u6a5f\u306b\u305d\u306e\u30c1\u30e5\u30fc\u30d6\u3092\u30bb\u30c3\u30c8\u3057\u3001\u906e\u5149\u3057\u30664\u6642\u9593\u4e7e\u71e5\u3092\u884c\u3046\u3002DMF\u304c\u84b8\u767a\u3057\u5207\u3063\u305f\u30c1\u30e5\u30fc\u30d6\u306b\u30ad\u30e3\u30c3\u30d7\u3092\u56fa\u304f\u3057\u3001\u906e\u5149\u3057\u305f\u30a2\u30eb\u30df\u888b\u306b\u4e7e\u71e5\u5264\u3068\u5171\u306b\u5165\u308c\u30664\u00b0C \u3067\u4fdd\u7ba1\u3059\u308b<\/li>\n\n\n\n
                    3. \u30ab\u30e9\u30e0\u306e\u30ad\u30e3\u30c3\u30d7\u306f\u9664\u53bb\u3057\u3066\u3057\u307e\u308f\u306a\u3044\u3053\u3068\u3002\u30ec\u30b8\u30f3\u304c\u4e7e\u3044\u3066\u3057\u307e\u3044\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30a8\u30eb\u30fc\u30b7\u30e7\u30f3\u91cf\u304c\u5909\u308f\u3063\u3066\u3057\u307e\u3046\u53ef\u80fd\u6027\u304c\u3042\u308b<\/li>\n\n\n\n
                    4. \u30b2\u30a4\u30f3\u7d71\u5408\u3092\u884c\u3048\u308b\u3088\u3046\u306b\u3001\u611f\u5ea6\u3092\u4f4e\u611f\u5ea6\u5074\u304b\u3089\u9ad8\u611f\u5ea6\u5074\u8fc4\u5e7e\u3064\u304b\u6761\u4ef6\u3092\u6307\u5b9a\u3057\u3066\u30b9\u30ad\u30e3\u30f3\u3057\u3066\u304a\u304f\u3053\u3068\u304c\u671b\u307e\u3057\u3044\uff085\u6761\u4ef6\u7a0b\u5ea6\uff09<\/li>\n\n\n\n
                    5. \u6297\u4f53\u30aa\u30fc\u30d0\u30fc\u30ec\u30a4\u6cd5\u306b\u95a2\u3059\u308b\u30aa\u30ea\u30b8\u30ca\u30eb\u8ad6\u6587\u3092\u53c2\u7167\uff08\u691c\u51fa\u306b\u7528\u3044\u308b\u6297\u4f53\u306e\u7cd6\u9396\u304c\u30ec\u30af\u30c1\u30f3\u3068\u53cd\u5fdc\u3059\u308b\u306e\u3092\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\uff09<\/a><\/li>\n<\/ol>\n\n\n\n

                      \u4ed8\u9332<\/h3>\n\n\n\n

                      \u30af\u30eb\u30fc\u30c9\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u62bd\u51fa\u306b\u3064\u3044\u3066\u306f\u3001Streptavidin coated magnetic beads\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u5bfe\u3059\u308b\u30d3\u30aa\u30c1\u30f3\u5316\u6297\u4f53\u3001\u305d\u3057\u3066TBS-Tx (TBS + TritonX-100 1% (v\/v))\u3092\u7528\u610f\u3057\u307e\u3059\u3002\u30d3\u30aa\u30c1\u30f3\u5316\u6297\u4f53\u3092streptavidin coated magnetic beads\u306b\u7d50\u5408\u3055\u305b\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30d3\u30aa\u30c1\u30f3\u5316\u6297\u4f53\u306b\u6355\u7372\u3055\u305b\u307e\u3059\u3002\u305d\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u300160\u00b0C \u30675\u5206\u9593\u306e\u71b1\u51e6\u7406\u3067\u3001\u30d3\u30aa\u30c1\u30f3\u5316\u6297\u4f53\u304b\u3089\u5916\u308c\u3001\u56de\u53ce\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30d3\u30aa\u30c1\u30f3\u3068\u30b9\u30c8\u30ec\u30d7\u30c8\u30a2\u30d3\u30b8\u30f3\u306e\u7d50\u5408\u306f\u5f37\u3044\u306e\u3067\u3001\u3053\u306e\u6761\u4ef6\u3067\u306f\u5916\u308c\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

                      \u6e96\u5099\u3059\u308b\u8a66\u85ac\u985e \u4f7f\u7528\u3059\u308b\u88c5\u7f6e \u30bf\u30f3\u30d1\u30af\u8cea\u306e\u62bd\u51fa \u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5b9a\u91cf Cy3\u306e\u30e9\u30d9\u30ea\u30f3\u30b0 \u30d5\u30ea\u30fc Cy3\u3092\u9664\u53bb\u3059\u308b\u305f\u3081\u306e\u30b2\u30eb\u308d\u904e\u30ab\u30e9\u30e0 \u30ec\u30af\u30c1\u30f3\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306b\u30a2\u30d7\u30e9\u30a4 \u30c7\u30fc\u30bf\u306e\u53d6\u5f97\u3068\u89e3\u6790\u6cd5 GlycoStation Rea<\/p><\/div>\n

                      \u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":5266,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-5399","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/pages\/5399","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/comments?post=5399"}],"version-history":[{"count":41,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/pages\/5399\/revisions"}],"predecessor-version":[{"id":14855,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/pages\/5399\/revisions\/14855"}],"up":[{"embeddable":true,"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/pages\/5266"}],"wp:attachment":[{"href":"https:\/\/www.emukk.com\/WP\/wp-json\/wp\/v2\/media?parent=5399"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}